Chapter title |
In Situ Demonstration and Characteristic Analysis of the Protease Using Substrate Immersing Zymography
|
---|---|
Chapter number | 19 |
Book title |
Zymography
|
Published in |
Methods in molecular biology, June 2017
|
DOI | 10.1007/978-1-4939-7111-4_19 |
Pubmed ID | |
Book ISBNs |
978-1-4939-7109-1, 978-1-4939-7111-4
|
Authors |
HaiLun He, Hao Li, Dan Liu |
Editors |
Jeff Wilkesman, Liliana Kurz |
Abstract |
Zymography, the detection of proteolytic activities on the basis of protein substrate degradation, has been a technique described in the literature for at least in the past 50 years. In this study, we used substrate immersing zymography to analyze proteolysis of proteases. Instead of being directly added into a sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) gel, the substrates were added into the immersing solution after electrophoresis. With substrate immersing zymography, some characters of proteases, such as enzyme forms, potential proteolytic activity, molecular weights, presence of complexes, and potentially active enzyme fragments in complex biological samples, can be determined. |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 4 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Bachelor | 2 | 50% |
Unknown | 2 | 50% |
Readers by discipline | Count | As % |
---|---|---|
Biochemistry, Genetics and Molecular Biology | 1 | 25% |
Engineering | 1 | 25% |
Unknown | 2 | 50% |