Chapter title |
Reverse Zymography: Overview and Pitfalls
|
---|---|
Chapter number | 11 |
Book title |
Zymography
|
Published in |
Methods in molecular biology, June 2017
|
DOI | 10.1007/978-1-4939-7111-4_11 |
Pubmed ID | |
Book ISBNs |
978-1-4939-7109-1, 978-1-4939-7111-4
|
Authors |
Kanika Sharma, Debasish Bhattacharyya |
Editors |
Jeff Wilkesman, Liliana Kurz |
Abstract |
Reverse zymography is a technique by which protease inhibitor(s) in a sample could be electrophoretically separated in a substrate-impregnated acrylamide gel and their relative abundance could be semi-quantified. The gel after electrophoresis is incubated with a protease when the impregnated substrate and all other proteins of the sample are degraded into small peptides except the inhibitor(s) that show clear bands against a white background. Since reverse zymography cannot distinguish between a protease inhibitor and a protein that is resistant against proteolysis, the results should be confirmed from inhibition of protease activity by solution state assay. |
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Researcher | 1 | 11% |
Unknown | 4 | 44% |
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