Chapter title |
Detection and Characterization of Bacterial Proteinases Using Zymography
|
---|---|
Chapter number | 9 |
Book title |
Zymography
|
Published in |
Methods in molecular biology, June 2017
|
DOI | 10.1007/978-1-4939-7111-4_9 |
Pubmed ID | |
Book ISBNs |
978-1-4939-7109-1, 978-1-4939-7111-4
|
Authors |
Madathiparambil G. Madanan, Ambili Mechoor |
Editors |
Jeff Wilkesman, Liliana Kurz |
Abstract |
Proteinases play a crucial role in invasion and pathogenesis of bacteria, especially the extracellular and membrane-bound forms. Analysis of these proteinases demands the isolation by retaining the enzymatic activity. The isolation procedures maintaining the native structure of the enzyme in its soluble form are also of extreme importance. The qualitative analyses of these proteinases are carried out by electrophoresis and zymography. Enzymatic characterization based on the effect of inhibitors and activators on gelatinase activity also can be assessed using this zymography. The membrane-bound proteinases can be isolated in their native and soluble form, still retaining the activity using 6-aminocaproic acid and sodium deoxycholate; the procedure of which is explained in this chapter. |
Mendeley readers
Geographical breakdown
Country | Count | As % |
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Unknown | 18 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
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Student > Bachelor | 4 | 22% |
Researcher | 3 | 17% |
Student > Doctoral Student | 2 | 11% |
Librarian | 1 | 6% |
Student > Master | 1 | 6% |
Other | 1 | 6% |
Unknown | 6 | 33% |
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Arts and Humanities | 1 | 6% |
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Chemistry | 1 | 6% |
Other | 0 | 0% |
Unknown | 9 | 50% |