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Zymography

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Cover of 'Zymography'

Table of Contents

  1. Altmetric Badge
    Book Overview
  2. Altmetric Badge
    Chapter 1 Zymography Principles
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    Chapter 2 Serine Protease Zymography: Low-Cost, Rapid, and Highly Sensitive RAMA Casein Zymography
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    Chapter 3 Cysteine Protease Zymography: Brief Review
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    Chapter 4 Aspartic Protease Zymography Case Study: Detection of Fungal Acid Proteases by Zymography
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    Chapter 5 Detection of Aspartic Proteinase Activities Using Gel Zymography
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    Chapter 6 MMP Activity Detection in Zymograms
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    Chapter 7 Characterization of Novel Collagenolytic Proteases
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    Chapter 8 Zymography as a Research Tool in the Study of Matrix Metalloproteinase Inhibitors
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    Chapter 9 Detection and Characterization of Bacterial Proteinases Using Zymography
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    Chapter 10 A Sensitive, Rapid, and Specific Technique for the Detection of Collagenase Using Zymography
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    Chapter 11 Reverse Zymography: Overview and Pitfalls
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    Chapter 12 Cell In Situ Zymography: Imaging Enzyme–Substrate Interactions
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    Chapter 13 Examination of Gelatinase Isoforms in Rodent Models of Acute Neurodegenerative Diseases Using Two-Dimensional Zymography
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    Chapter 14 Two-Dimensional Zymography of Proteases from Steatotic Duck Liver
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    Chapter 15 Simultaneous Detection of Activity and Relative Molecular Mass of Adenylate Kinases After SDS-PAGE and Blotting
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    Chapter 16 Silver-Stained Fibrin Zymography: Separation of Proteases and Activity Detection Using a Single Substrate-Containing Gel
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    Chapter 17 Zymography Methods to Simultaneously Analyze Superoxide Dismutase and Catalase Activities: Novel Application for Yeast Species Identification
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    Chapter 18 Detection of Guaiacol Peroxidase on Electrophoretic Gels
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    Chapter 19 In Situ Demonstration and Characteristic Analysis of the Protease Using Substrate Immersing Zymography
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    Chapter 20 Use of Zymography in Trypanosomiasis Studies
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    Chapter 21 Zymography in Multiwells for Quality Assessment of Proteinases
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    Chapter 22 Visualization of Enzyme Activities in Earthworm Biopores by In Situ Soil Zymography
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    Chapter 23 Multiplex Cathepsin Zymography to Detect Amounts of Active Cathepsins K, L, S, and V
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    Chapter 24 Transfer Zymography
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    Chapter 25 Sequential Detection of Thermophilic Lipase and Protease by Zymography
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    Chapter 26 Calpain Zymography: General Methodology and Protocol
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    Chapter 27 CTAB Zymography for the Analysis of Aspartic Proteases from Marine Sponges
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    Chapter 28 Zymography Detection of a Bacterial Extracellular Thermoalkaline Esterase/Lipase Activity
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    Chapter 29 Amylase Zymography
Attention for Chapter 17: Zymography Methods to Simultaneously Analyze Superoxide Dismutase and Catalase Activities: Novel Application for Yeast Species Identification
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Chapter title
Zymography Methods to Simultaneously Analyze Superoxide Dismutase and Catalase Activities: Novel Application for Yeast Species Identification
Chapter number 17
Book title
Methods in Molecular Biology
Published in
Methods in molecular biology, June 2017
DOI 10.1007/978-1-4939-7111-4_17
Pubmed ID
Book ISBNs
978-1-4939-7109-1, 978-1-4939-7111-4
Authors

Gamero-Sandemetrio, Esther, Gómez-Pastor, Rocío, Matallana, Emilia, Esther Gamero-Sandemetrio, Rocío Gómez-Pastor, Emilia Matallana

Editors

Jeff Wilkesman, Liliana Kurz

Abstract

We provide an optimized protocol for a double staining technique to analyze superoxide dismutase enzymatic isoforms Cu-Zn SOD (Sod1) and Mn-SOD (Sod2) and catalase in the same polyacrylamide gel. The use of NaCN, which specifically inhibits yeast Sod1 isoform, allows the analysis of Sod2 isoform while the use of H2O2 allows the analysis of catalase. The identification of a different zymography profiling of SOD and catalase isoforms in different yeast species allowed us to propose this technique as a novel yeast identification and classification strategy.

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The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 4 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 4 100%

Demographic breakdown

Readers by professional status Count As %
Researcher 3 75%
Unknown 1 25%
Readers by discipline Count As %
Agricultural and Biological Sciences 2 50%
Biochemistry, Genetics and Molecular Biology 1 25%
Unknown 1 25%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 12 July 2017.
All research outputs
#18,555,330
of 22,981,247 outputs
Outputs from Methods in molecular biology
#7,946
of 13,148 outputs
Outputs of similar age
#242,150
of 317,529 outputs
Outputs of similar age from Methods in molecular biology
#182
of 301 outputs
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