Chapter title |
ChIP-seq for the Identification of Functional Elements in the Human Genome
|
---|---|
Chapter number | 1 |
Book title |
Promoter Associated RNA
|
Published in |
Methods in molecular biology, March 2017
|
DOI | 10.1007/978-1-4939-6716-2_1 |
Pubmed ID | |
Book ISBNs |
978-1-4939-6714-8, 978-1-4939-6716-2
|
Authors |
Marinov, Georgi K., Georgi K. Marinov |
Editors |
Sara Napoli |
Abstract |
Functional elements in the genome express their function through physical association with particular proteins: transcription factors, components of the transcription machinery, specific histone modifications, and others. The genome-wide characterization of the protein-DNA interaction landscape of these proteins is thus a key approach toward the identification of candidate genomic regulatory regions. ChIP-seq (Chromatin Immunoprecipitation coupled with high-throughput sequencing) has emerged as the primary experimental methods for carrying out this task. Here, the ChIP-seq protocol is described together with some of the most important considerations for applying it in practice. |
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