Chapter title |
Assessment of Respiration in Isolated Plant Mitochondria Using Clark-Type Electrodes
|
---|---|
Chapter number | 12 |
Book title |
Plant Mitochondria
|
Published in |
Methods in molecular biology, January 2015
|
DOI | 10.1007/978-1-4939-2639-8_12 |
Pubmed ID | |
Book ISBNs |
978-1-4939-2638-1, 978-1-4939-2639-8
|
Authors |
Richard P. Jacoby, A. Harvey Millar, Nicolas L. Taylor |
Editors |
James Whelan, Monika W. Murcha |
Abstract |
Mitochondrial respiration involves two key gas exchanges, the consumption of oxygen and the release of carbon dioxide. The ability to measure the consumption of oxygen via Clark-type electrodes has been one of the key techniques for advancing our knowledge of mitochondrial function in whole organisms, tissue samples, cells, and isolated subcellular fractions. In plants, oxygen electrode analyses provided the first evidence for some of the unique respiratory properties of plant mitochondria. This chapter briefs the principles of respiration and oxidative phosphorylation, how oxygen consumption measurements can be used to assess the quality of isolated mitochondrial preparations, and how these measurements can answer important questions in plant biochemistry and physiology. Finally, it presents instructions on assembling the oxygen electrode apparatus and how to conduct various assays. |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 23 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Ph. D. Student | 8 | 35% |
Student > Bachelor | 6 | 26% |
Student > Doctoral Student | 2 | 9% |
Student > Master | 2 | 9% |
Professor | 1 | 4% |
Other | 3 | 13% |
Unknown | 1 | 4% |
Readers by discipline | Count | As % |
---|---|---|
Agricultural and Biological Sciences | 12 | 52% |
Biochemistry, Genetics and Molecular Biology | 5 | 22% |
Unspecified | 1 | 4% |
Nursing and Health Professions | 1 | 4% |
Environmental Science | 1 | 4% |
Other | 2 | 9% |
Unknown | 1 | 4% |