| Chapter title |
Engineering Well-Characterized PEG-Coated Nanoparticles for Elucidating Biological Barriers to Drug Delivery
|
|---|---|
| Chapter number | 8 |
| Book title |
Cancer Nanotechnology
|
| Published in |
Methods in molecular biology, February 2017
|
| DOI | 10.1007/978-1-4939-6646-2_8 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-6644-8, 978-1-4939-6646-2
|
| Authors |
Qi Yang, Samuel K. Lai |
| Editors |
Reema Zeineldin |
| Abstract |
Poly(ethylene glycol) (PEG) coatings can substantially reduce nanoparticle uptake and clearance by immune cells as well as nonspecific interactions with the biological environment, thus potentially improving nanoparticle circulation times and biodistribution in target tissues such as tumors. Naturally, the "stealth" properties of PEG coatings are critically dependent on the density and conformation of surface PEG chains. However, there are significant technical hurdles to both generating sufficiently dense PEG coatings on nanoparticles and precisely characterizing their PEG grafting densities. Here, we describe methods for preparing PEGylated polymeric nanoparticles with precisely tunable PEG coatings without the use of organic solvents, quantifying PEGylation efficiency and density using a standard fluorescence assay, and evaluating nanoparticle uptake by immune cells using flow cytometry. |
Mendeley readers
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 15 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 4 | 27% |
| Student > Bachelor | 3 | 20% |
| Student > Master | 2 | 13% |
| Student > Doctoral Student | 1 | 7% |
| Student > Postgraduate | 1 | 7% |
| Other | 0 | 0% |
| Unknown | 4 | 27% |
| Readers by discipline | Count | As % |
|---|---|---|
| Engineering | 5 | 33% |
| Pharmacology, Toxicology and Pharmaceutical Science | 3 | 20% |
| Medicine and Dentistry | 1 | 7% |
| Biochemistry, Genetics and Molecular Biology | 1 | 7% |
| Unknown | 5 | 33% |