Chapter title |
Histochemical Demonstration of Tripeptidyl Aminopeptidase I
|
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Chapter number | 4 |
Book title |
Histochemistry of Single Molecules
|
Published in |
Methods in molecular biology, February 2017
|
DOI | 10.1007/978-1-4939-6788-9_4 |
Pubmed ID | |
Book ISBNs |
978-1-4939-6787-2, 978-1-4939-6788-9
|
Authors |
Mashenka B. Dimitrova, Dimitrinka Y. Atanasova, Nikolai E. Lazarov |
Editors |
Carlo Pellicciari, Marco Biggiogera |
Abstract |
Enzyme histochemical methods are valuable for the studies on the enzyme involvement in different pathological processes. Here we describe two protocols for chromogenic and fluorogenic histochemical demonstration of tripeptidyl aminopeptidase I (TPPI), a protease that is crucial for neuronal functions. The procedures are based on newly synthesized substrates for TPPI-glycyl-L-prolyl-L-metionyl-5-chloro-1-anthraquinonylhydrazide (GPM-CAH) and glycyl-L-prolyl-L-metionyl-4-hydrazido-N-hexyl-1,8-naphthalimide (GPM-HHNI). Using such protocols, precise enzyme localization can be obtained in tissue sections of mammalian organs. |