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Chapter title |
Enzyme-Histochemistry Technique for Visualizing the Dipeptidyl-Peptidase IV (DPP-IV) Activity in the Liver Biliary Tree
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Chapter number | 3 |
Book title |
Histochemistry of Single Molecules
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Published in |
Methods in molecular biology, February 2017
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DOI | 10.1007/978-1-4939-6788-9_3 |
Pubmed ID | |
Book ISBNs |
978-1-4939-6787-2, 978-1-4939-6788-9
|
Authors |
Vittorio Bertone, Eleonora Tarantola, Isabel Freitas |
Editors |
Carlo Pellicciari, Marco Biggiogera |
Abstract |
Dipeptidyl-peptidase IV is an enzyme involved in a lot of biochemical processes, where it modifies a number of regulatory proteins by removing the terminal peptides by hydrolysis. Here we describe a histochemical method to demonstrate with accuracy and precision its in situ activity on cryostatic section of Wistar rat liver by means of a simultaneous azo-coupling method. |