You are seeing a free-to-access but limited selection of the activity Altmetric has collected about this research output.
Click here to find out more.
Mendeley readers
Chapter title |
Heat-Induced Antigen Retrieval in Immunohistochemistry: Mechanisms and Applications
|
---|---|
Chapter number | 10 |
Book title |
Histochemistry of Single Molecules
|
Published in |
Methods in molecular biology, February 2017
|
DOI | 10.1007/978-1-4939-6788-9_10 |
Pubmed ID | |
Book ISBNs |
978-1-4939-6787-2, 978-1-4939-6788-9
|
Authors |
Shuji Yamashita, Osamu Katsumata |
Editors |
Carlo Pellicciari, Marco Biggiogera |
Abstract |
Heat-induced antigen retrieval (HIAR) is an essential technique for current immunohistochemistry. We describe the mechanisms of HIAR and its protocols for formalin-fixed, paraffin-embedded specimens and frozen sections, which are the most popular materials for immunohistochemistry using light microscopy. In addition, we describe the antigen retrieval method for highly masked epitopes and double immunostaining using the horseradish peroxidase (HRP)-labeled antibody method and immunofluorescence method in this chapter. |
Mendeley readers
The data shown below were compiled from readership statistics for 20 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 20 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Bachelor | 4 | 20% |
Student > Ph. D. Student | 4 | 20% |
Student > Master | 3 | 15% |
Student > Doctoral Student | 3 | 15% |
Other | 1 | 5% |
Other | 2 | 10% |
Unknown | 3 | 15% |
Readers by discipline | Count | As % |
---|---|---|
Medicine and Dentistry | 5 | 25% |
Biochemistry, Genetics and Molecular Biology | 5 | 25% |
Agricultural and Biological Sciences | 2 | 10% |
Pharmacology, Toxicology and Pharmaceutical Science | 1 | 5% |
Neuroscience | 1 | 5% |
Other | 0 | 0% |
Unknown | 6 | 30% |