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Mendeley readers
Chapter title |
DNA Labeling at Electron Microscopy
|
---|---|
Chapter number | 20 |
Book title |
Histochemistry of Single Molecules
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Published in |
Methods in molecular biology, February 2017
|
DOI | 10.1007/978-1-4939-6788-9_20 |
Pubmed ID | |
Book ISBNs |
978-1-4939-6787-2, 978-1-4939-6788-9
|
Authors |
Nicolas Thelen, Marc Thiry |
Editors |
Carlo Pellicciari, Marco Biggiogera |
Abstract |
Here, we describe a method for locating DNA on ultrathin sections. This technique is compatible with all usual fixation and embedding procedures and can be combined with cytochemical methods. Ultrathin sections are incubated in a medium containing terminal deoxynucleotidyl transferase (TdT) and various non-isotopic nucleotide analogs. The labeled nucleotides bound to the surface of ultrathin sections are then visualized by an indirect immunogold labeling technique. This high-resolution method provides a powerful tool for pinpointing the precise location of DNA within biological material, even where DNA is present in very low amounts. |
Mendeley readers
The data shown below were compiled from readership statistics for 4 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 4 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Researcher | 2 | 50% |
Student > Bachelor | 1 | 25% |
Unknown | 1 | 25% |
Readers by discipline | Count | As % |
---|---|---|
Agricultural and Biological Sciences | 2 | 50% |
Pharmacology, Toxicology and Pharmaceutical Science | 1 | 25% |
Unknown | 1 | 25% |