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Chapter title |
Nile Red Staining of Neutral Lipids in Yeast
|
---|---|
Chapter number | 16 |
Book title |
Histochemistry of Single Molecules
|
Published in |
Methods in molecular biology, February 2017
|
DOI | 10.1007/978-1-4939-6788-9_16 |
Pubmed ID | |
Book ISBNs |
978-1-4939-6787-2, 978-1-4939-6788-9
|
Authors |
Kerry Ann Rostron, Clare Louise Lawrence, Rostron, Kerry Ann, Lawrence, Clare Louise |
Editors |
Carlo Pellicciari, Marco Biggiogera |
Abstract |
Determination of cellular neutral lipid levels in yeast is important for both the biotechnology industry and biomedical research. However, many of the currently available methods are labor intensive and time consuming. Here we describe a rapid and repeatable method for the detection of neutral lipids, which can be utilized in both oleaginous and non-oleaginous yeast species. The method utilizes the fluorescent dye, Nile red, which enables neutral lipid levels to either be visualized via microscopy or quantified using a 96-well plate assay. |
Mendeley readers
The data shown below were compiled from readership statistics for 43 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 43 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Ph. D. Student | 11 | 26% |
Researcher | 6 | 14% |
Student > Master | 6 | 14% |
Student > Bachelor | 3 | 7% |
Professor | 1 | 2% |
Other | 0 | 0% |
Unknown | 16 | 37% |
Readers by discipline | Count | As % |
---|---|---|
Biochemistry, Genetics and Molecular Biology | 13 | 30% |
Agricultural and Biological Sciences | 9 | 21% |
Chemical Engineering | 2 | 5% |
Environmental Science | 1 | 2% |
Immunology and Microbiology | 1 | 2% |
Other | 1 | 2% |
Unknown | 16 | 37% |