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Light Microscopy

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Cover of 'Light Microscopy'

Table of Contents

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    Book Overview
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    Chapter 1 Introduction to Modern Methods in Light Microscopy
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    Chapter 2 Three-Dimensional Live Imaging of Filamentous Fungi with Light Sheet-Based Fluorescence Microscopy (LSFM)
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    Chapter 3 Light-Sheet Fluorescence Microscopy: Chemical Clearing and Labeling Protocols for Ultramicroscopy
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    Chapter 4 Two-Photon Intravital Microscopy Animal Preparation Protocol to Study Cellular Dynamics in Pathogenesis
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    Chapter 5 Imaging of Brain Slices with a Genetically Encoded Voltage Indicator
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    Chapter 6 FRET Microscopy for Real-Time Visualization of Second Messengers in Living Cells
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    Chapter 7 Imaging the Dynamics of Cell Wall Polymer Deposition in the Unicellular Model Plant, Penium margaritaceum
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    Chapter 8 Targeted Ablation Using Laser Nanosurgery
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    Chapter 9 Sample Preparation and Choice of Fluorophores for Single and Dual Color Photo-Activated Localization Microscopy (PALM) with Bacterial Cells
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    Chapter 10 STED Imaging in Drosophila Brain Slices
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    Chapter 11 Two-Color Total Internal Reflection Fluorescence Microscopy of Exocytosis in Endocrine Cells
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    Chapter 12 Optical Coherence Microscopy
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    Chapter 13 Designing Image Analysis Pipelines in Light Microscopy: A Rational Approach
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    Chapter 14 Automated Analysis of Intracellular Dynamic Processes
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    Chapter 15 Quantitative Image Analysis of Single-Molecule mRNA Dynamics in Living Cells
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    Chapter 16 Analysis of Protein Kinetics Using Fluorescence Recovery After Photobleaching (FRAP)
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    Chapter 17 Fluorescence-Based High-Throughput and Targeted Image Acquisition and Analysis for Phenotypic Screening
Attention for Chapter 17: Fluorescence-Based High-Throughput and Targeted Image Acquisition and Analysis for Phenotypic Screening
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Chapter title
Fluorescence-Based High-Throughput and Targeted Image Acquisition and Analysis for Phenotypic Screening
Chapter number 17
Book title
Light Microscopy
Published in
Methods in molecular biology, March 2017
DOI 10.1007/978-1-4939-6810-7_17
Pubmed ID
Book ISBNs
978-1-4939-6808-4, 978-1-4939-6810-7
Authors

Manuel Gunkel, Jan Philipp Eberle, Holger Erfle

Editors

Yolanda Markaki, Hartmann Harz

Abstract

Applying the right acquisition method in a fluorescence imaging-based screening context is of great importance to obtain an appropriate readout and to select the right scale of the screen. In order to save imaging time and data, we have developed routines for multiscale targeted imaging, providing both a broad overview of a sample and additional in-depth information for targets of interest identified within the screen. These objects can be identified and acquired on-the-fly by an interconnection of image acquisition and image analysis.

Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 2 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 2 100%

Demographic breakdown

Readers by professional status Count As %
Professor 1 50%
Unknown 1 50%
Readers by discipline Count As %
Pharmacology, Toxicology and Pharmaceutical Science 1 50%
Unknown 1 50%