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Light Microscopy

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Cover of 'Light Microscopy'

Table of Contents

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    Book Overview
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    Chapter 1 Introduction to Modern Methods in Light Microscopy
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    Chapter 2 Three-Dimensional Live Imaging of Filamentous Fungi with Light Sheet-Based Fluorescence Microscopy (LSFM)
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    Chapter 3 Light-Sheet Fluorescence Microscopy: Chemical Clearing and Labeling Protocols for Ultramicroscopy
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    Chapter 4 Two-Photon Intravital Microscopy Animal Preparation Protocol to Study Cellular Dynamics in Pathogenesis
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    Chapter 5 Imaging of Brain Slices with a Genetically Encoded Voltage Indicator
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    Chapter 6 FRET Microscopy for Real-Time Visualization of Second Messengers in Living Cells
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    Chapter 7 Imaging the Dynamics of Cell Wall Polymer Deposition in the Unicellular Model Plant, Penium margaritaceum
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    Chapter 8 Targeted Ablation Using Laser Nanosurgery
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    Chapter 9 Sample Preparation and Choice of Fluorophores for Single and Dual Color Photo-Activated Localization Microscopy (PALM) with Bacterial Cells
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    Chapter 10 STED Imaging in Drosophila Brain Slices
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    Chapter 11 Two-Color Total Internal Reflection Fluorescence Microscopy of Exocytosis in Endocrine Cells
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    Chapter 12 Optical Coherence Microscopy
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    Chapter 13 Designing Image Analysis Pipelines in Light Microscopy: A Rational Approach
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    Chapter 14 Automated Analysis of Intracellular Dynamic Processes
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    Chapter 15 Quantitative Image Analysis of Single-Molecule mRNA Dynamics in Living Cells
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    Chapter 16 Analysis of Protein Kinetics Using Fluorescence Recovery After Photobleaching (FRAP)
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    Chapter 17 Fluorescence-Based High-Throughput and Targeted Image Acquisition and Analysis for Phenotypic Screening
Attention for Chapter 1: Introduction to Modern Methods in Light Microscopy
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Chapter title
Introduction to Modern Methods in Light Microscopy
Chapter number 1
Book title
Light Microscopy
Published in
Methods in molecular biology, March 2017
DOI 10.1007/978-1-4939-6810-7_1
Pubmed ID
Book ISBNs
978-1-4939-6808-4, 978-1-4939-6810-7
Authors

Joel Ryan, Abby R. Gerhold, Vincent Boudreau, Lydia Smith, Paul S. Maddox, Ryan, Joel, Gerhold, Abby R., Boudreau, Vincent, Smith, Lydia, Maddox, Paul S.

Editors

Yolanda Markaki, Hartmann Harz

Abstract

For centuries, light microscopy has been a key method in biological research, from the early work of Robert Hooke describing biological organisms as cells, to the latest in live-cell and single-molecule systems. Here, we introduce some of the key concepts related to the development and implementation of modern microscopy techniques. We briefly discuss the basics of optics in the microscope, super-resolution imaging, quantitative image analysis, live-cell imaging, and provide an outlook on active research areas pertaining to light microscopy.

Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 53 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 53 100%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 10 19%
Student > Master 9 17%
Student > Bachelor 4 8%
Student > Doctoral Student 2 4%
Professor 2 4%
Other 6 11%
Unknown 20 38%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 9 17%
Engineering 5 9%
Agricultural and Biological Sciences 5 9%
Immunology and Microbiology 4 8%
Medicine and Dentistry 3 6%
Other 5 9%
Unknown 22 42%