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Chapter title |
Three-Dimensional Live Imaging of Filamentous Fungi with Light Sheet-Based Fluorescence Microscopy (LSFM)
|
---|---|
Chapter number | 2 |
Book title |
Light Microscopy
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Published in |
Methods in molecular biology, March 2017
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DOI | 10.1007/978-1-4939-6810-7_2 |
Pubmed ID | |
Book ISBNs |
978-1-4939-6808-4, 978-1-4939-6810-7
|
Authors |
Francesco Pampaloni, Laura Knuppertz, Andrea Hamann, Heinz D. Osiewacz, Ernst H. K. Stelzer |
Editors |
Yolanda Markaki, Hartmann Harz |
Abstract |
We describe a method for the three-dimensional live imaging of filamentous fungi with light sheet-based fluorescence microscopy (LSFM). LSFM provides completely new opportunities to investigate the biology of fungal cells and other microorganisms with high spatial and temporal resolution. As an example, we study the established aging model Podospora anserina. The protocol explains the mounting of the live fungi for the light sheet imaging, the imaging procedure and illustrates basic image processing of data. |
Mendeley readers
The data shown below were compiled from readership statistics for 7 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 7 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Other | 1 | 14% |
Student > Doctoral Student | 1 | 14% |
Professor | 1 | 14% |
Student > Ph. D. Student | 1 | 14% |
Researcher | 1 | 14% |
Other | 0 | 0% |
Unknown | 2 | 29% |
Readers by discipline | Count | As % |
---|---|---|
Physics and Astronomy | 2 | 29% |
Earth and Planetary Sciences | 1 | 14% |
Agricultural and Biological Sciences | 1 | 14% |
Unknown | 3 | 43% |