| Chapter title |
SlipChip Device for Digital Nucleic Acid Amplification.
|
|---|---|
| Chapter number | 10 |
| Book title |
Microchip Diagnostics
|
| Published in |
Methods in molecular biology, January 2017
|
| DOI | 10.1007/978-1-4939-6734-6_10 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-6732-2, 978-1-4939-6734-6
|
| Authors |
Feng Shen |
| Editors |
Valérie Taly, Jean-Louis Viovy, Stéphanie Descroix |
| Abstract |
Digital nucleic acid amplification (Digital NAA) quantifies nucleic acid by compartmentalizing a sample of DNA or RNA into a large number of discrete partitions and performing parallel nucleic acid amplification, such as polymerase chain reaction (PCR) or isothermal amplification reactions. With the counts of positive wells, total number of wells, and volumes of wells, the concentration of the target nucleic acid in the sample can be quantified. Digital NAA is considered increasingly powerful for ultra-sensitive detection and accurate quantification of nucleic acid for biological research and potentially medical diagnostics. Here, we describe glass SlipChip devices to perform digital NAA without cumbersome manual manipulation or complex fluidic control systems. |
Mendeley readers
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 7 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 2 | 29% |
| Unspecified | 1 | 14% |
| Student > Bachelor | 1 | 14% |
| Student > Doctoral Student | 1 | 14% |
| Unknown | 2 | 29% |
| Readers by discipline | Count | As % |
|---|---|---|
| Chemical Engineering | 2 | 29% |
| Unspecified | 1 | 14% |
| Chemistry | 1 | 14% |
| Engineering | 1 | 14% |
| Unknown | 2 | 29% |