| Chapter title |
Capillary-Driven Microfluidic Chips for Miniaturized Immunoassays: Patterning Capture Antibodies Using Microcontact Printing and Dry-Film Resists.
|
|---|---|
| Chapter number | 3 |
| Book title |
Microchip Diagnostics
|
| Published in |
Methods in molecular biology, January 2017
|
| DOI | 10.1007/978-1-4939-6734-6_3 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-6732-2, 978-1-4939-6734-6
|
| Authors |
Yuksel Temiz, Robert D. Lovchik, Emmanuel Delamarche |
| Editors |
Valérie Taly, Jean-Louis Viovy, Stéphanie Descroix |
| Abstract |
The miniaturization of immunoassays using microfluidic devices is attractive for many applications, but an important challenge remains the patterning of capture antibodies (cAbs) on the surface of microfluidic structures. Here, we describe how to pattern cAbs on planar poly(dimethylsiloxane) (PDMS) stamps and how to microcontact print the cAbs on a dry-film resist (DFR). DFRs are new types of photoresists having excellent chemical resistance and good mechanical, adhesive, and optical properties. Instead of being liquid photoresists, DFRs are thin layers that are easy to handle, cut, photo-pattern, and laminate over surfaces. We show how to perform a simple fluorescence immunoassay using anti-biotin cAbs patterned on a 50-μm-thick DF-1050 DFR, Atto 647N-biotin analytes, and capillary-driven chips fabricated in silicon. |
Mendeley readers
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 12 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 3 | 25% |
| Student > Ph. D. Student | 3 | 25% |
| Student > Doctoral Student | 3 | 25% |
| Unspecified | 1 | 8% |
| Student > Master | 1 | 8% |
| Other | 0 | 0% |
| Unknown | 1 | 8% |
| Readers by discipline | Count | As % |
|---|---|---|
| Engineering | 7 | 58% |
| Chemical Engineering | 1 | 8% |
| Physics and Astronomy | 1 | 8% |
| Unspecified | 1 | 8% |
| Unknown | 2 | 17% |