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Chapter title |
Fabrication and Evaluation of Microfluidic Immunoassay Devices with Antibody-Immobilized Microbeads Retained in Porous Hydrogel Micropillars.
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Chapter number | 4 |
Book title |
Microchip Diagnostics
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Published in |
Methods in molecular biology, January 2017
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DOI | 10.1007/978-1-4939-6734-6_4 |
Pubmed ID | |
Book ISBNs |
978-1-4939-6732-2, 978-1-4939-6734-6
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Authors |
Toshihiro Kasama, Noritada Kaji, Manabu Tokeshi, Yoshinobu Baba |
Editors |
Valérie Taly, Jean-Louis Viovy, Stéphanie Descroix |
Abstract |
Due to the inherent characteristics including confinement of molecular diffusion and high surface-to-volume ratio, microfluidic device-based immunoassay has great advantages in cost, speed, sensitivity, and so on, compared with conventional techniques such as microtiter plate-based ELISA, latex agglutination method, and lateral flow immunochromatography. In this paper, we explain the detection of C-reactive protein as a model antigen by using our microfluidic immunoassay device, so-called immuno-pillar device. We describe in detail how we fabricated and used the immuno-pillar devices. |
Mendeley readers
The data shown below were compiled from readership statistics for 18 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 18 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Researcher | 7 | 39% |
Professor | 3 | 17% |
Student > Master | 2 | 11% |
Student > Doctoral Student | 1 | 6% |
Student > Ph. D. Student | 1 | 6% |
Other | 0 | 0% |
Unknown | 4 | 22% |
Readers by discipline | Count | As % |
---|---|---|
Immunology and Microbiology | 4 | 22% |
Engineering | 3 | 17% |
Agricultural and Biological Sciences | 3 | 17% |
Business, Management and Accounting | 1 | 6% |
Chemical Engineering | 1 | 6% |
Other | 0 | 0% |
Unknown | 6 | 33% |