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Microchip Diagnostics

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Cover of 'Microchip Diagnostics'

Table of Contents

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    Book Overview
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    Chapter 1 Microfluidics-Enabled Diagnostic Systems: Markets, Challenges, and Examples.
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    Chapter 2 Capillary-Driven Microfluidic Chips for Miniaturized Immunoassays: Efficient Fabrication and Sealing of Chips Using a "Chip-Olate" Process.
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    Chapter 3 Capillary-Driven Microfluidic Chips for Miniaturized Immunoassays: Patterning Capture Antibodies Using Microcontact Printing and Dry-Film Resists.
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    Chapter 4 Fabrication and Evaluation of Microfluidic Immunoassay Devices with Antibody-Immobilized Microbeads Retained in Porous Hydrogel Micropillars.
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    Chapter 5 Using Electrophoretic Immunoassay to Monitor Hormone Secretion.
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    Chapter 6 On-Chip Magnetic Particle-Based Immunoassays Using Multilaminar Flow for Clinical Diagnostics.
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    Chapter 7 Digital Microfluidics Assisted Sealing of Individual Magnetic Particles in Femtoliter-Sized Reaction Wells for Single-Molecule Detection.
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    Chapter 8 Microfluidic DNA Stretching Device for Single-Molecule Diagnostics.
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    Chapter 9 Droplet Microfluidic and Magnetic Particles Platform for Cancer Typing.
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    Chapter 10 SlipChip Device for Digital Nucleic Acid Amplification.
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    Chapter 11 Multiplex Detection of KRAS Mutations Using Passive Droplet Fusion.
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    Chapter 12 Droplet-Based Microfluidics Digital PCR for the Detection of KRAS Mutations.
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    Chapter 13 Negative Enrichment of Circulating Tumor Cells in Blood Using a Microfluidic Chip.
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    Chapter 14 Microfluidic-Based Bacteria Isolation from Whole Blood for Diagnostics of Blood Stream Infection.
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    Chapter 15 Microfluidic Platform for Parallel Single Cell Analysis for Diagnostic Applications.
  17. Altmetric Badge
    Chapter 16 FISH-in-CHIPS: A Microfluidic Platform for Molecular Typing of Cancer Cells.
Attention for Chapter 4: Fabrication and Evaluation of Microfluidic Immunoassay Devices with Antibody-Immobilized Microbeads Retained in Porous Hydrogel Micropillars.
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Chapter title
Fabrication and Evaluation of Microfluidic Immunoassay Devices with Antibody-Immobilized Microbeads Retained in Porous Hydrogel Micropillars.
Chapter number 4
Book title
Microchip Diagnostics
Published in
Methods in molecular biology, January 2017
DOI 10.1007/978-1-4939-6734-6_4
Pubmed ID
Book ISBNs
978-1-4939-6732-2, 978-1-4939-6734-6
Authors

Toshihiro Kasama, Noritada Kaji, Manabu Tokeshi, Yoshinobu Baba

Editors

Valérie Taly, Jean-Louis Viovy, Stéphanie Descroix

Abstract

Due to the inherent characteristics including confinement of molecular diffusion and high surface-to-volume ratio, microfluidic device-based immunoassay has great advantages in cost, speed, sensitivity, and so on, compared with conventional techniques such as microtiter plate-based ELISA, latex agglutination method, and lateral flow immunochromatography. In this paper, we explain the detection of C-reactive protein as a model antigen by using our microfluidic immunoassay device, so-called immuno-pillar device. We describe in detail how we fabricated and used the immuno-pillar devices.

Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 18 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 18 100%

Demographic breakdown

Readers by professional status Count As %
Researcher 7 39%
Professor 3 17%
Student > Master 2 11%
Student > Doctoral Student 1 6%
Student > Ph. D. Student 1 6%
Other 0 0%
Unknown 4 22%
Readers by discipline Count As %
Immunology and Microbiology 4 22%
Engineering 3 17%
Agricultural and Biological Sciences 3 17%
Business, Management and Accounting 1 6%
Chemical Engineering 1 6%
Other 0 0%
Unknown 6 33%