| Chapter title |
Microfluidic DNA Stretching Device for Single-Molecule Diagnostics.
|
|---|---|
| Chapter number | 8 |
| Book title |
Microchip Diagnostics
|
| Published in |
Methods in molecular biology, January 2017
|
| DOI | 10.1007/978-1-4939-6734-6_8 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-6732-2, 978-1-4939-6734-6
|
| Authors |
Daisuke Onoshima, Yoshinobu Baba |
| Editors |
Valérie Taly, Jean-Louis Viovy, Stéphanie Descroix |
| Abstract |
The method described here enables the automatic stretching and patterning of single DNA molecules onto a solid surface. It does not require chemical modification of the DNA or surface modification of the substrate. To detect a signal variation caused by sequence-specific dye binding or partial melting, it is crucial that the DNA molecules are arrayed in a parallel direction inside the narrow microscopic field. The method uses zigzag-shaped microgrooves in a densely-arranged molecular patterning apparatus in a microfluidic channel. By syringing through the microchannel, over 1500 DNA molecules can be arrayed simultaneously in the microgrooves. It will therefore serve as a template preparation for DNA molecular diagnosis by high-resolution imaging. |
Mendeley readers
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 4 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 2 | 50% |
| Professor | 1 | 25% |
| Unknown | 1 | 25% |
| Readers by discipline | Count | As % |
|---|---|---|
| Chemical Engineering | 1 | 25% |
| Engineering | 1 | 25% |
| Unknown | 2 | 50% |