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Chapter title |
Monosynaptic Tracing in Developing Circuits Using Modified Rabies Virus.
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Chapter number | 23 |
Book title |
Synapse Development
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Published in |
Methods in molecular biology, January 2017
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DOI | 10.1007/978-1-4939-6688-2_23 |
Pubmed ID | |
Book ISBNs |
978-1-4939-6686-8, 978-1-4939-6688-2
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Authors |
Laura Cocas, Gloria Fernandez |
Editors |
Alexandros Poulopoulos |
Abstract |
An attenuated rabies virus that expresses fluorescent protein has made it possible to analyze retrograde (presynaptic) monosynaptic connections in vivo. By combining attenuated rabies virus with a Cre-loxP based system to target cells in a subtype-specific fashion, it is possible to examine neuronal input in vivo onto any class of neuron, in development and in the mature brain. We describe here the methods to amplify deletion mutant, pseudotyped rabies virus, selectively target cells of interest using genetic and viral approaches, as well as the stereotaxic procedures required to target neuronal subtypes of interest in vivo. |
Mendeley readers
The data shown below were compiled from readership statistics for 5 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 5 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Ph. D. Student | 2 | 40% |
Researcher | 1 | 20% |
Student > Postgraduate | 1 | 20% |
Student > Master | 1 | 20% |
Readers by discipline | Count | As % |
---|---|---|
Agricultural and Biological Sciences | 2 | 40% |
Veterinary Science and Veterinary Medicine | 1 | 20% |
Pharmacology, Toxicology and Pharmaceutical Science | 1 | 20% |
Neuroscience | 1 | 20% |