Chapter title |
Analyzing Structural Plasticity of Dendritic Spines in Organotypic Slice Culture.
|
---|---|
Chapter number | 19 |
Book title |
Synapse Development
|
Published in |
Methods in molecular biology, January 2017
|
DOI | 10.1007/978-1-4939-6688-2_19 |
Pubmed ID | |
Book ISBNs |
978-1-4939-6686-8, 978-1-4939-6688-2
|
Authors |
Mathias De Roo, Adema Ribic |
Editors |
Alexandros Poulopoulos |
Abstract |
Understanding the rules of synapse dynamics in the context of development, learning, and nervous system disorders is an important part of several fields of neuroscience. Despite significant methodological advances, observations of structural dynamics of synapses still present a significant experimental challenge. In this chapter we describe a set of techniques that allow repetitive observations of synaptic structures in vitro in organotypic cultures of rodent hippocampus. We describe culturing of slices, transfection with reporter-carrying plasmids, repetitive imaging of dendritic spines with confocal laser scanning microscopy and analysis of spine morphology dynamics. |
Mendeley readers
Geographical breakdown
Country | Count | As % |
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Unknown | 13 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Researcher | 5 | 38% |
Other | 2 | 15% |
Student > Ph. D. Student | 1 | 8% |
Librarian | 1 | 8% |
Student > Master | 1 | 8% |
Other | 1 | 8% |
Unknown | 2 | 15% |
Readers by discipline | Count | As % |
---|---|---|
Neuroscience | 5 | 38% |
Agricultural and Biological Sciences | 2 | 15% |
Social Sciences | 1 | 8% |
Biochemistry, Genetics and Molecular Biology | 1 | 8% |
Medicine and Dentistry | 1 | 8% |
Other | 1 | 8% |
Unknown | 2 | 15% |