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Mendeley readers
Chapter title |
In Situ Protein Binding Assay Using Fc-Fusion Proteins.
|
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Chapter number | 7 |
Book title |
Synapse Development
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Published in |
Methods in molecular biology, January 2017
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DOI | 10.1007/978-1-4939-6688-2_7 |
Pubmed ID | |
Book ISBNs |
978-1-4939-6686-8, 978-1-4939-6688-2
|
Authors |
Nirmala Padmanabhan, Tabrez J. Siddiqui |
Editors |
Alexandros Poulopoulos |
Abstract |
This protocol describes an in situ protein-protein interaction assay between tagged recombinant proteins and cell-surface expressed synaptic proteins. The assay is arguably more sensitive than other traditional protein binding assays such as co-immunoprecipitation and pull-downs and provides a visual readout for binding. This assay has been widely used to determine the dissociation constant of binding of trans-synaptic adhesion proteins. The step-wise description in the protocol should facilitate the adoption of this method in other laboratories. |
Mendeley readers
The data shown below were compiled from readership statistics for 2 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 2 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Ph. D. Student | 1 | 50% |
Unknown | 1 | 50% |
Readers by discipline | Count | As % |
---|---|---|
Immunology and Microbiology | 1 | 50% |
Unknown | 1 | 50% |