Chapter title |
Dual Anterograde and Retrograde Viral Tracing of Reciprocal Connectivity.
|
---|---|
Chapter number | 21 |
Book title |
Synapse Development
|
Published in |
Methods in molecular biology, January 2017
|
DOI | 10.1007/978-1-4939-6688-2_21 |
Pubmed ID | |
Book ISBNs |
978-1-4939-6686-8, 978-1-4939-6688-2
|
Authors |
Matthias G. Haberl, Melanie Ginger, Andreas Frick Ph.D., Andreas Frick |
Editors |
Alexandros Poulopoulos |
Abstract |
Current large-scale approaches in neuroscience aim to unravel the complete connectivity map of specific neuronal circuits, or even the entire brain. This emerging research discipline has been termed connectomics. Recombinant glycoprotein-deleted rabies virus (RABV ∆G) has become an important tool for the investigation of neuronal connectivity in the brains of a variety of species. Neuronal infection with even a single RABV ∆G particle results in high-level transgene expression, revealing the fine-detailed morphology of all neuronal features-including dendritic spines, axonal processes, and boutons-on a brain-wide scale. This labeling is eminently suitable for subsequent post-hoc morphological analysis, such as semiautomated reconstruction in 3D. Here we describe the use of a recently developed anterograde RABV ∆G variant together with a retrograde RABV ∆G for the investigation of projections both to, and from, a particular brain region. In addition to the automated reconstruction of a dendritic tree, we also give as an example the volume measurements of axonal boutons following RABV ∆G-mediated fluorescent marker expression. In conclusion RABV ∆G variants expressing a combination of markers and/or tools for stimulating/monitoring neuronal activity, used together with genetic or behavioral animal models, promise important insights in the structure-function relationship of neural circuits. |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 17 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Researcher | 4 | 24% |
Student > Postgraduate | 3 | 18% |
Student > Master | 2 | 12% |
Student > Ph. D. Student | 2 | 12% |
Librarian | 1 | 6% |
Other | 2 | 12% |
Unknown | 3 | 18% |
Readers by discipline | Count | As % |
---|---|---|
Neuroscience | 6 | 35% |
Agricultural and Biological Sciences | 2 | 12% |
Medicine and Dentistry | 2 | 12% |
Biochemistry, Genetics and Molecular Biology | 1 | 6% |
Veterinary Science and Veterinary Medicine | 1 | 6% |
Other | 1 | 6% |
Unknown | 4 | 24% |