Chapter title |
Metabolomic Analysis of Campylobacter jejuni by Direct-Injection Electrospray Ionization Mass Spectrometry.
|
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Chapter number | 16 |
Book title |
Campylobacter jejuni
|
Published in |
Methods in molecular biology, January 2017
|
DOI | 10.1007/978-1-4939-6536-6_16 |
Pubmed ID | |
Book ISBNs |
978-1-4939-6534-2, 978-1-4939-6536-6
|
Authors |
Robert M. Howlett, Matthew P. Davey, David J. Kelly, Howlett, Robert M, Davey, Matthew P, Kelly, David J, Howlett, Robert M., Davey, Matthew P., Kelly, David J. |
Editors |
James Butcher, Alain Stintzi |
Abstract |
Direct-injection mass spectrometry (DIMS) is a means of rapidly obtaining metabolomic phenotype data in both prokaryotes and eukaryotes. Given our generally poor understanding of Campylobacter metabolism, the high-throughput and relatively simple sample preparation of DIMS has made this an attractive technique for metabolism-related studies and hypothesis generation, especially when attempting to analyze metabolic mutants with no clear phenotype. Here we describe a metabolomic fingerprinting approach with sampling and extraction methodologies optimized for direct-injection electrospray ionization mass spectrometry (ESI-MS), which we have used as a means of comparing wild-type and isogenic mutant strains of C. jejuni with various metabolic blocks. |
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