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Campylobacter jejuni

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Cover of 'Campylobacter jejuni'

Table of Contents

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    Book Overview
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    Chapter 1 Campylobacter jejuni Isolation/Enumeration from Environmental Samples.
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    Chapter 2 Recovery of Campylobacter spp. from Food and Environmental Sources.
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    Chapter 3 Methods for Isolation, Purification, and Propagation of Bacteriophages of Campylobacter jejuni.
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    Chapter 4 Methods to Study Antimicrobial Resistance in Campylobacter jejuni.
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    Chapter 5 Method of Peptide Nucleic Acid (PNA)-Mediated Antisense Inhibition of Gene Expression in Campylobacter jejuni.
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    Chapter 6 Identification of Ligand-Receptor Interactions: Ligand Molecular Arrays, SPR and NMR Methodologies.
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    Chapter 7 Characterization of High Affinity Iron Acquisition Systems in Campylobacter jejuni.
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    Chapter 8 Method for the Successful Crystallization of the Ferric Uptake Regulator from Campylobacter jejuni.
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    Chapter 9 Methods for Initial Characterization of Campylobacter jejuni Bacteriophages.
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    Chapter 10 Methods to Assess the Direct Interaction of C. jejuni with Mucins.
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    Chapter 11 Methods to Study Campylobacter jejuni Adherence to and Invasion of Host Epithelial Cells.
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    Chapter 12 Assays to Study the Interaction of Campylobacter jejuni with the Mucosal Surface.
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    Chapter 13 Characterization of Ligand-Receptor Interactions: Chemotaxis, Biofilm, Cell Culture Assays, and Animal Model Methodologies.
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    Chapter 14 Using Galleria mellonella as an Infection Model for Campylobacter jejuni Pathogenesis.
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    Chapter 15 Mouse Models for Campylobacter jejuni Colonization and Infection.
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    Chapter 16 Metabolomic Analysis of Campylobacter jejuni by Direct-Injection Electrospray Ionization Mass Spectrometry.
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    Chapter 17 Methods for Genome-Wide Methylome Profiling of Campylobacter jejuni.
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    Chapter 18 Characterizing Glycoproteins by Mass Spectrometry in Campylobacter jejuni.
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    Chapter 19 Methods for Proteome Analysis of Campylobacter jejuni Using 2-D Electrophoresis.
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    Chapter 20 Analyzing Prokaryotic RNA-Seq Data: A Case Study Identifying Holo-Fur Regulated Genes in Campylobacter jejuni.
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    Chapter 21 Generation and Screening of an Insertion Sequencing-Compatible Mutant Library of Campylobacter jejuni.
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    Chapter 22 Erratum to: Assays to Study the Interaction of Campylobacter jejuni with the Mucosal Surface
Attention for Chapter 13: Characterization of Ligand-Receptor Interactions: Chemotaxis, Biofilm, Cell Culture Assays, and Animal Model Methodologies.
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Chapter title
Characterization of Ligand-Receptor Interactions: Chemotaxis, Biofilm, Cell Culture Assays, and Animal Model Methodologies.
Chapter number 13
Book title
Campylobacter jejuni
Published in
Methods in molecular biology, January 2017
DOI 10.1007/978-1-4939-6536-6_13
Pubmed ID
Book ISBNs
978-1-4939-6534-2, 978-1-4939-6536-6
Authors

Rebecca M. King, Victoria Korolik

Editors

James Butcher, Alain Stintzi

Abstract

Chemotactic motility is an essential virulence factor for the pathogenesis of Campylobacter spp. infection. In Chapter 6 , we described technologies that enable initial screening and identification of ligands able to interact with chemoreceptor sensory domains. These include amino acid and glycan arrays, NMR, and SPR that are utilized to identify potential ligands interacting with Campylobacter jejuni. Here we describe techniques that enable the characterization and evaluation of ligand-receptor binding in chemotaxis through the assessment of motility and directed chemotactic motility as well as the associated phenotypes-autoagglutination behavior, biofilm formation, ability to adhere and invade cultured mammalian cells, and colonization ability in avian hosts.

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X Demographics

The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 11 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 11 100%

Demographic breakdown

Readers by professional status Count As %
Researcher 2 18%
Student > Ph. D. Student 1 9%
Professor 1 9%
Student > Master 1 9%
Student > Postgraduate 1 9%
Other 0 0%
Unknown 5 45%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 2 18%
Veterinary Science and Veterinary Medicine 1 9%
Materials Science 1 9%
Medicine and Dentistry 1 9%
Unknown 6 55%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 11 January 2018.
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#20,355,479
of 22,903,988 outputs
Outputs from Methods in molecular biology
#9,922
of 13,133 outputs
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#355,337
of 420,462 outputs
Outputs of similar age from Methods in molecular biology
#845
of 1,074 outputs
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