You are seeing a free-to-access but limited selection of the activity Altmetric has collected about this research output.
Click here to find out more.
Mendeley readers
Chapter title |
Methods for Isolation, Purification, and Propagation of Bacteriophages of Campylobacter jejuni.
|
---|---|
Chapter number | 3 |
Book title |
Campylobacter jejuni
|
Published in |
Methods in molecular biology, January 2017
|
DOI | 10.1007/978-1-4939-6536-6_3 |
Pubmed ID | |
Book ISBNs |
978-1-4939-6534-2, 978-1-4939-6536-6
|
Authors |
Yilmaz Emre Gencay, Tina Birk, Martine Camilla Holst Sørensen, Lone Brøndsted |
Editors |
James Butcher, Alain Stintzi |
Abstract |
Here, we describe the methods for isolation, purification, and propagation of Campylobacter jejuni bacteriophages from samples expected to contain high number of phages such as chicken feces. The overall steps are (1) liberation of phages from the sample material; (2) observation of plaque-forming units on C. jejuni lawns using a spot assay; (3) isolation of single plaques; (4) consecutive purification procedures; and (5) propagation of purified phages from a plate lysate to prepare master stocks. |
Mendeley readers
The data shown below were compiled from readership statistics for 60 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 60 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Master | 9 | 15% |
Student > Bachelor | 8 | 13% |
Researcher | 6 | 10% |
Student > Ph. D. Student | 5 | 8% |
Student > Postgraduate | 4 | 7% |
Other | 8 | 13% |
Unknown | 20 | 33% |
Readers by discipline | Count | As % |
---|---|---|
Biochemistry, Genetics and Molecular Biology | 9 | 15% |
Agricultural and Biological Sciences | 6 | 10% |
Immunology and Microbiology | 4 | 7% |
Veterinary Science and Veterinary Medicine | 3 | 5% |
Engineering | 3 | 5% |
Other | 10 | 17% |
Unknown | 25 | 42% |