Chapter title |
Characterization of High Affinity Iron Acquisition Systems in Campylobacter jejuni.
|
---|---|
Chapter number | 7 |
Book title |
Campylobacter jejuni
|
Published in |
Methods in molecular biology, January 2017
|
DOI | 10.1007/978-1-4939-6536-6_7 |
Pubmed ID | |
Book ISBNs |
978-1-4939-6534-2, 978-1-4939-6536-6
|
Authors |
Ximin Zeng, Jun Lin |
Editors |
James Butcher, Alain Stintzi |
Abstract |
Iron acquisition systems are critical for bacterial pathogenesis and thus have been proposed as attractive targets for iron-dependent pathogen control. Of these systems, high-affinity iron acquisition mediated by siderophore, a small iron chelator, is the most efficient iron-scavenging mechanism in gram-negative bacteria. Campylobacter does not produce any siderophores but has the ability to utilize exogenous siderophores. In particular, the enterobactin (Ent)-mediated iron scavenging is tightly linked to Campylobacter pathogenesis. To date, Ent, a triscatecholate with the highest known affinity for ferric iron, is a well-characterized siderophore used by Campylobacter for iron acquisition during in vivo infection. Here, we describe the key methods used to characterize Ent-mediated high affinity iron acquisition system in Campylobacter jejuni. |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 7 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
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Researcher | 2 | 29% |
Student > Bachelor | 1 | 14% |
Student > Master | 1 | 14% |
Unknown | 3 | 43% |
Readers by discipline | Count | As % |
---|---|---|
Biochemistry, Genetics and Molecular Biology | 2 | 29% |
Pharmacology, Toxicology and Pharmaceutical Science | 1 | 14% |
Nursing and Health Professions | 1 | 14% |
Unknown | 3 | 43% |