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Chapter title |
Methods to Assess the Direct Interaction of C. jejuni with Mucins.
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Chapter number | 10 |
Book title |
Campylobacter jejuni
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Published in |
Methods in molecular biology, January 2017
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DOI | 10.1007/978-1-4939-6536-6_10 |
Pubmed ID | |
Book ISBNs |
978-1-4939-6534-2, 978-1-4939-6536-6
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Authors |
Marguerite Clyne, Gina Duggan, Julie Naughton, Billy Bourke, Clyne, Marguerite, Duggan, Gina, Naughton, Julie, Bourke, Billy |
Editors |
James Butcher, Alain Stintzi |
Abstract |
Studies of the interaction of bacteria with mucus-secreting cells can be complemented at a more mechanistic level by exploring the interaction of bacteria with purified mucins. Here we describe a far Western blotting approach to show how C. jejuni proteins separated by SDS PAGE and transferred to a membrane or slot blotted directly onto a membrane can be probed using biotinylated mucin. In addition we describe the use of novel mucin microarrays to assess bacterial interactions with mucins in a high-throughput manner. |
Mendeley readers
The data shown below were compiled from readership statistics for 7 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 7 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Researcher | 2 | 29% |
Student > Master | 1 | 14% |
Unknown | 4 | 57% |
Readers by discipline | Count | As % |
---|---|---|
Veterinary Science and Veterinary Medicine | 1 | 14% |
Engineering | 1 | 14% |
Unknown | 5 | 71% |