| Chapter title |
A 3D Fibroblast-Epithelium Co-culture Model for Understanding Microenvironmental Role in Branching Morphogenesis of the Mammary Gland.
|
|---|---|
| Chapter number | 10 |
| Book title |
Mammary Gland Development
|
| Published in |
Methods in molecular biology, January 2017
|
| DOI | 10.1007/978-1-4939-6475-8_10 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-6473-4, 978-1-4939-6475-8
|
| Authors |
Zuzana Koledova, Pengfei Lu |
| Editors |
Finian Martin, Torsten Stein, Jillian Howlin |
| Abstract |
The mammary gland consists of numerous tissue compartments, including mammary epithelium, an array of stromal cells, and the extracellular matrix (ECM). Bidirectional interactions between the epithelium and its surrounding stroma are essential for proper mammary gland development and homeostasis, whereas their deregulation leads to developmental abnormalities and cancer. To study the relationships between the epithelium and the stroma, development of models that could recapitulate essential aspects of these interacting systems in vitro has become necessary. Here we describe a three-dimensional (3D) co-culture assay and show that the addition of fibroblasts to mammary organoid cultures promotes the epithelium to undergo branching morphogenesis, thus allowing the role of the stromal microenvironment to be examined in this essential developmental process. |
Mendeley readers
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 34 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 5 | 15% |
| Student > Ph. D. Student | 4 | 12% |
| Student > Doctoral Student | 3 | 9% |
| Other | 3 | 9% |
| Student > Bachelor | 2 | 6% |
| Other | 5 | 15% |
| Unknown | 12 | 35% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 7 | 21% |
| Medicine and Dentistry | 4 | 12% |
| Agricultural and Biological Sciences | 3 | 9% |
| Chemical Engineering | 2 | 6% |
| Engineering | 2 | 6% |
| Other | 3 | 9% |
| Unknown | 13 | 38% |