Contractility Assay for Established Myoepithelial Cell Lines.
Mammary Gland Development
Methods in molecular biology, January 2017
Stéphanie Cagnet, Marina A. Glukhova, Karine Raymond
Finian Martin, Torsten Stein, Jillian Howlin
The capacity of mammary myoepithelial cells to contract in response to suckling stimuli is essential for lactation. We describe here a protocol for studying the contractile activity of myoepithelial cells in vitro. This protocol includes the establishment of stable myoepithelial cell lines from mouse mammary glands and quantitative evaluation of the contraction and subsequent relaxation of cultured myoepithelial cells in response to oxytocin. It can be used for analyses of mouse mutants with gene deletions or overexpression altering myoepithelial cell function.
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