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Wnt Signaling

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Cover of 'Wnt Signaling'

Table of Contents

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    Book Overview
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    Chapter 1 Visualizing Wnt Palmitoylation in Single Cells.
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    Chapter 2 Monitoring Wnt Protein Acylation Using an In Vitro Cyclo-Addition Reaction.
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    Chapter 3 Biochemical Methods to Analyze Wnt Protein Secretion.
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    Chapter 4 Methods for Studying Wnt Protein Modifications/Inactivations by Extracellular Enzymes, Tiki and Notum.
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    Chapter 5 Probing Wnt Receptor Turnover: A Critical Regulatory Point of Wnt Pathway.
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    Chapter 6 A Simple Method to Assess Abundance of the β-Catenin Signaling Pool in Cells.
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    Chapter 7 Wnt-Dependent Control of Cell Polarity in Cultured Cells.
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    Chapter 8 The Use of Chick Embryos to Study Wnt Activity Gradients.
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    Chapter 9 Monitoring Wnt Signaling in Zebrafish Using Fluorescent Biosensors.
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    Chapter 10 Wnt Signaling
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    Chapter 11 Wnt Signaling
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    Chapter 12 Delivery of the Porcupine Inhibitor WNT974 in Mice.
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    Chapter 13 Use of Primary Calvarial Osteoblasts to Evaluate the Function of Wnt Signaling in Osteogenesis.
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    Chapter 14 Monitoring Wnt/β-Catenin Signaling in Skin.
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    Chapter 15 The Generation of Organoids for Studying Wnt Signaling.
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    Chapter 16 Methods to Manipulate and Monitor Wnt Signaling in Human Pluripotent Stem Cells.
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    Chapter 17 Wnt Signaling
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    Chapter 18 Erratum to: Delivery of the Porcupine Inhibitor WNT974 in Mice
Attention for Chapter 15: The Generation of Organoids for Studying Wnt Signaling.
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Chapter title
The Generation of Organoids for Studying Wnt Signaling.
Chapter number 15
Book title
Wnt Signaling
Published in
Methods in molecular biology, January 2016
DOI 10.1007/978-1-4939-6393-5_15
Pubmed ID
Book ISBNs
978-1-4939-6391-1, 978-1-4939-6393-5
Authors

Jarno Drost, Benedetta Artegiani, Hans Clevers

Editors

Quinn Barrett, Lawrence Lum

Abstract

We established an in vitro culture model in which intestinal epithelial stem cells can grow into three-dimensional, ever-expanding epithelial organoids that retain their original organ identity and genetic stability. Moreover, organoids can easily be genetically modified using different genome modification strategies, including viral delivery of transgenes and CRISPR/Cas9 technology. These combined characteristics make them a useful in vitro model system to study many biological processes including the contribution of cellular signaling pathways to tissue homeostasis and disease. Here we describe our current laboratory protocols to establish human intestinal organoids and how to genetically modify both mouse and human intestinal organoids to study cellular signaling pathways, specifically Wnt signaling. Moreover, we provide a detailed protocol for lentiviral transduction and CRISPR/Cas9-mediated genome modification of organoid cultures.

Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 68 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Sweden 1 1%
Germany 1 1%
Unknown 66 97%

Demographic breakdown

Readers by professional status Count As %
Researcher 15 22%
Student > Bachelor 14 21%
Student > Ph. D. Student 11 16%
Student > Master 6 9%
Student > Doctoral Student 3 4%
Other 7 10%
Unknown 12 18%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 21 31%
Agricultural and Biological Sciences 15 22%
Medicine and Dentistry 6 9%
Chemistry 3 4%
Immunology and Microbiology 3 4%
Other 7 10%
Unknown 13 19%