| Chapter title |
Monitoring Wnt Signaling in Zebrafish Using Fluorescent Biosensors.
|
|---|---|
| Chapter number | 9 |
| Book title |
Wnt Signaling
|
| Published in |
Methods in molecular biology, January 2016
|
| DOI | 10.1007/978-1-4939-6393-5_9 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-6391-1, 978-1-4939-6393-5
|
| Authors |
Nicola Facchinello, Marco Schiavone, Andrea Vettori, Francesco Argenton, Natascia Tiso |
| Editors |
Quinn Barrett, Lawrence Lum |
| Abstract |
In this chapter, we are presenting methods to monitor and quantify in vivo canonical Wnt signaling activities at single-cell resolution in zebrafish. Our technology is based on artificial enhancers, obtained by polymerization of TCF binding elements, cloned upstream to ubiquitous or tissue-specific promoters. The different promoter/enhancer combinations are used to drive fluorescent protein reporter constructs integrated in the zebrafish germline by microinjection of fertilized zebrafish eggs. Fish with a single integration site are selected by Mendelian analysis of fluorescent carriers, and heterozygous offspring are used to monitor and quantify canonical Wnt activities. Open source public domain software such as ImageJ/Fiji is used to calculate the integrated densities in the region of interest and compare the effect of experimental conditions on control and treated animals. |
Mendeley readers
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|---|---|---|
| Unknown | 17 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 7 | 41% |
| Student > Ph. D. Student | 3 | 18% |
| Professor | 1 | 6% |
| Student > Bachelor | 1 | 6% |
| Student > Postgraduate | 1 | 6% |
| Other | 0 | 0% |
| Unknown | 4 | 24% |
| Readers by discipline | Count | As % |
|---|---|---|
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| Agricultural and Biological Sciences | 2 | 12% |
| Neuroscience | 1 | 6% |
| Unknown | 6 | 35% |