| Chapter title |
Monitoring Wnt/β-Catenin Signaling in Skin.
|
|---|---|
| Chapter number | 14 |
| Book title |
Wnt Signaling
|
| Published in |
Methods in molecular biology, January 2016
|
| DOI | 10.1007/978-1-4939-6393-5_14 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-6391-1, 978-1-4939-6393-5
|
| Authors |
Amy T. Ku, Qi Miao, Hoang Nguyen |
| Editors |
Quinn Barrett, Lawrence Lum |
| Abstract |
Wnt signaling through β-catenin plays a crucial role in skin development and homeostasis. Disruption or hyperactivation of this pathway results in skin defects and diseases (Lim and Nusse, Cold Spring Harb Perspect Biol 5(2), 2013). Monitoring Wnt signaling in skin under normal and abnormal conditions is therefore critical to understand the role of this pathway in development and homeostasis.In this chapter, we provide methods to detect Wnt/β-catenin (canonical) signaling in the skin. We present a comprehensive list of Wnt reporter mice and detail the processing of skin tissue to detect reporter genes. From this list, we focus on the three most recent lines that, according to reports, are the most sensitive in skin. Additionally, we describe a protocol to detect nuclear β-catenin, a hallmark of active Wnt signaling, although this technique should be used with caution due to its limited sensitivity. The techniques outlined below will be useful for detecting active Wnt signaling in skin. |
Mendeley readers
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 15 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 3 | 20% |
| Student > Bachelor | 2 | 13% |
| Student > Ph. D. Student | 2 | 13% |
| Student > Doctoral Student | 1 | 7% |
| Unspecified | 1 | 7% |
| Other | 1 | 7% |
| Unknown | 5 | 33% |
| Readers by discipline | Count | As % |
|---|---|---|
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| Medicine and Dentistry | 3 | 20% |
| Business, Management and Accounting | 1 | 7% |
| Unspecified | 1 | 7% |
| Agricultural and Biological Sciences | 1 | 7% |
| Other | 1 | 7% |
| Unknown | 4 | 27% |