| Chapter title |
Methods to Manipulate and Monitor Wnt Signaling in Human Pluripotent Stem Cells.
|
|---|---|
| Chapter number | 16 |
| Book title |
Wnt Signaling
|
| Published in |
Methods in molecular biology, January 2016
|
| DOI | 10.1007/978-1-4939-6393-5_16 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-6391-1, 978-1-4939-6393-5
|
| Authors |
Ian J. Huggins, David Brafman, Karl Willert |
| Editors |
Quinn Barrett, Lawrence Lum |
| Abstract |
Human pluripotent stem cells (hPSCs) may revolutionize medical practice by providing: (a) a renewable source of cells for tissue replacement therapies, (b) a powerful system to model human diseases in a dish, and (c) a platform for examining efficacy and safety of novel drugs. Furthermore, these cells offer a unique opportunity to study early human development in vitro, in particular, the process by which a seemingly uniform cell population interacts to give rise to the three main embryonic lineages: ectoderm, endoderm. and mesoderm. This process of lineage allocation is regulated by a number of inductive signals that are mediated by growth factors, including FGF, TGFβ, and Wnt. In this book chapter, we introduce a set of tools, methods, and protocols to specifically manipulate the Wnt signaling pathway with the intention of altering the cell fate outcome of hPSCs. |
Mendeley readers
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 10 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Bachelor | 2 | 20% |
| Researcher | 2 | 20% |
| Student > Ph. D. Student | 1 | 10% |
| Unspecified | 1 | 10% |
| Student > Master | 1 | 10% |
| Other | 0 | 0% |
| Unknown | 3 | 30% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 4 | 40% |
| Medicine and Dentistry | 2 | 20% |
| Psychology | 1 | 10% |
| Unspecified | 1 | 10% |
| Unknown | 2 | 20% |