| Chapter title |
The Physcomitrella patens System for Transient Gene Expression Assays
|
|---|---|
| Chapter number | 10 |
| Book title |
Plant Synthetic Promoters
|
| Published in |
Methods in molecular biology, January 2016
|
| DOI | 10.1007/978-1-4939-6396-6_10 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-6394-2, 978-1-4939-6396-6
|
| Authors |
Johanne Thévenin, Wenjia Xu, Louise Vaisman, Loïc Lepiniec, Bertrand Dubreucq, Christian Dubos |
| Editors |
Reinhard Hehl |
| Abstract |
Transient expression assays are valuable techniques to study in vivo the transcriptional regulation of gene expression. These methods allow to assess the transcriptional properties of a given transcription factor (TF) or a complex of regulatory proteins against specific DNA motifs, called cis-regulatory elements. Here, we describe a fast, efficient, and reliable method based on the use of Physcomitrella patens protoplasts that allows the study of gene expression in a qualitative and quantitative manner by combining the advantage of GFP (green fluorescent protein) as a marker of promoter activity with flow cytometry for accurate measurement of fluorescence in individual cells. |
X Demographics
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Australia | 1 | 33% |
| Unknown | 2 | 67% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 3 | 100% |
Mendeley readers
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 16 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 5 | 31% |
| Student > Doctoral Student | 3 | 19% |
| Student > Ph. D. Student | 1 | 6% |
| Student > Bachelor | 1 | 6% |
| Student > Master | 1 | 6% |
| Other | 1 | 6% |
| Unknown | 4 | 25% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 6 | 38% |
| Agricultural and Biological Sciences | 6 | 38% |
| Unknown | 4 | 25% |