| Chapter title |
Spatio-Temporal Imaging of Promoter Activity in Intact Plant Tissues
|
|---|---|
| Chapter number | 7 |
| Book title |
Plant Synthetic Promoters
|
| Published in |
Methods in molecular biology, January 2016
|
| DOI | 10.1007/978-1-4939-6396-6_7 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-6394-2, 978-1-4939-6396-6
|
| Authors |
Tou Cheu Xiong, Frédéric Sanchez, Jean-François Briat, Frédéric Gaymard, Christian Dubos |
| Editors |
Reinhard Hehl |
| Abstract |
Localization and quantification of expression levels of genes help to determine their function. Localization of gene expression is often achieved through the study of their promoter activity. Three main reporter genes β-glucuronidase (GUS), green fluorescent protein (GFP), and luciferase (LUC) have been intensively used to characterize promoter activities, each having its own specificities and advantages. Among them, the LUC reporter gene is best suitable for the analysis of the promoter activity of genes in intact living plants. Here, we describe a LUC-based method that allows to precisely localize and quantify promoter activity at the whole plant level, and to study the mechanisms that are involved in long-distance regulation of gene expression in Arabidopsis thaliana. Imaging LUC signals with a low-light CCD camera allows monitoring promoter activity in time and space in the transgenic plant harboring the promoter fused with the LUC gene. In addition, it allows quantifying change of promoter activities in plant during several hours. |
Mendeley readers
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 5 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Doctoral Student | 2 | 40% |
| Researcher | 1 | 20% |
| Student > Ph. D. Student | 1 | 20% |
| Unknown | 1 | 20% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 4 | 80% |
| Unknown | 1 | 20% |