| Chapter title |
Simultaneous Analysis of Multiple Promoters: An Application of the PC-GW Binary Vector Series
|
|---|---|
| Chapter number | 13 |
| Book title |
Plant Synthetic Promoters
|
| Published in |
Methods in molecular biology, January 2016
|
| DOI | 10.1007/978-1-4939-6396-6_13 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-6394-2, 978-1-4939-6396-6
|
| Authors |
Jyoti Dalal Ph.D., Jyoti Dalal |
| Editors |
Reinhard Hehl |
| Abstract |
With the advances in the field of synthetic biology, there is an increasing demand for multi-gene cloning technologies. Molecular cloning to generate multi-gene constructs can be performed by restriction digestion, or by recombination-based cloning strategies such as Gateway(®). This chapter details cloning, transformation, and selection procedures involved in generation of multi-gene expressing transgenic plants. Methods are described for cloning five distinct promoter-reporter fusion constructs into the PC-GW-BAR vector (from the PC-GW vector series) using Gateway(®) technology and meganuclease sites. Further, transformation and selection methods are described for the biofuel crop Camelina sativa from the Brassicaceae family. These methods would be constructive toward generating multi-gene expressing plants for simultaneous expression analysis of five promoters in a short time period. |
Mendeley readers
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| China | 1 | 17% |
| Unknown | 5 | 83% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 3 | 50% |
| Student > Master | 1 | 17% |
| Unknown | 2 | 33% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 3 | 50% |
| Biochemistry, Genetics and Molecular Biology | 1 | 17% |
| Unknown | 2 | 33% |