| Chapter title |
Quantitative Analysis of Cis-Regulatory Element Activity Using Synthetic Promoters in Transgenic Plants
|
|---|---|
| Chapter number | 2 |
| Book title |
Plant Synthetic Promoters
|
| Published in |
Methods in molecular biology, January 2016
|
| DOI | 10.1007/978-1-4939-6396-6_2 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-6394-2, 978-1-4939-6396-6
|
| Authors |
Geoffrey Benn, Katayoon Dehesh |
| Editors |
Reinhard Hehl |
| Abstract |
Synthetic promoters, introduced stably or transiently into plants, are an invaluable tool for the identification of functional regulatory elements and the corresponding transcription factor(s) that regulate the amplitude, spatial distribution, and temporal patterns of gene expression. Here, we present a protocol describing the steps required to identify and characterize putative cis-regulatory elements. These steps include application of computational tools to identify putative elements, construction of a synthetic promoter upstream of luciferase, identification of transcription factors that regulate the element, testing the functionality of the element introduced transiently and/or stably into the species of interest followed by high-throughput luciferase screening assays, and subsequent data processing and statistical analysis. |
Mendeley readers
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United States | 1 | 11% |
| Unknown | 8 | 89% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 2 | 22% |
| Researcher | 2 | 22% |
| Student > Doctoral Student | 1 | 11% |
| Other | 1 | 11% |
| Student > Master | 1 | 11% |
| Other | 1 | 11% |
| Unknown | 1 | 11% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 5 | 56% |
| Biochemistry, Genetics and Molecular Biology | 3 | 33% |
| Unknown | 1 | 11% |