Chapter title |
FootprintDB: Analysis of Plant Cis-Regulatory Elements, Transcription Factors, and Binding Interfaces
|
---|---|
Chapter number | 17 |
Book title |
Plant Synthetic Promoters
|
Published in |
Methods in molecular biology, January 2016
|
DOI | 10.1007/978-1-4939-6396-6_17 |
Pubmed ID | |
Book ISBNs |
978-1-4939-6394-2, 978-1-4939-6396-6
|
Authors |
Bruno Contreras-Moreira, Alvaro Sebastian, Contreras-Moreira, Bruno, Sebastian, Alvaro |
Editors |
Reinhard Hehl |
Abstract |
FootprintDB is a database and search engine that compiles regulatory sequences from open access libraries of curated DNA cis-elements and motifs, and their associated transcription factors (TFs). It systematically annotates the binding interfaces of the TFs by exploiting protein-DNA complexes deposited in the Protein Data Bank. Each entry in footprintDB is thus a DNA motif linked to the protein sequence of the TF(s) known to recognize it, and in most cases, the set of predicted interface residues involved in specific recognition. This chapter explains step-by-step how to search for DNA motifs and protein sequences in footprintDB and how to focus the search to a particular organism. Two real-world examples are shown where this software was used to analyze transcriptional regulation in plants. Results are described with the aim of guiding users on their interpretation, and special attention is given to the choices users might face when performing similar analyses. |
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