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Mendeley readers
| Chapter title |
Viral Injection and Cranial Window Implantation for In Vivo Two-Photon Imaging.
|
|---|---|
| Chapter number | 10 |
| Book title |
High-Resolution Imaging of Cellular Proteins
|
| Published in |
Methods in molecular biology, January 2016
|
| DOI | 10.1007/978-1-4939-6352-2_10 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-6350-8, 978-1-4939-6352-2
|
| Authors |
Gordon B. Smith, David Fitzpatrick |
| Editors |
Steven D. Schwartzbach, Omar Skalli, Thomas Schikorski |
| Abstract |
Two-photon (2P) imaging has proven to be a powerful tool for investigating neural structure and function both in brain slices and in intact systems. In vivo 2P imaging presents significant challenges in sample preparation, which are exacerbated in non-murine species. Here, we describe procedures for the effective virally mediated labeling of neurons and for the implantation of cranial windows for imaging. The procedures described here are applicable to a range of species, including mice, and are routinely used in ferrets and tree shrews to provide large-scale labeling of cortical volumes and high-quality imaging data. |
Mendeley readers
The data shown below were compiled from readership statistics for 23 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United Kingdom | 1 | 4% |
| Unknown | 22 | 96% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 8 | 35% |
| Student > Ph. D. Student | 5 | 22% |
| Student > Master | 3 | 13% |
| Student > Bachelor | 1 | 4% |
| Student > Doctoral Student | 1 | 4% |
| Other | 0 | 0% |
| Unknown | 5 | 22% |
| Readers by discipline | Count | As % |
|---|---|---|
| Neuroscience | 8 | 35% |
| Agricultural and Biological Sciences | 4 | 17% |
| Engineering | 2 | 9% |
| Medicine and Dentistry | 2 | 9% |
| Physics and Astronomy | 1 | 4% |
| Other | 0 | 0% |
| Unknown | 6 | 26% |