| Chapter title |
Helper-Dependent Adenoviral Vectors and Their Use for Neuroscience Applications.
|
|---|---|
| Chapter number | 5 |
| Book title |
High-Resolution Imaging of Cellular Proteins
|
| Published in |
Methods in molecular biology, January 2016
|
| DOI | 10.1007/978-1-4939-6352-2_5 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-6350-8, 978-1-4939-6352-2
|
| Authors |
Mónica S. Montesinos, Rachel Satterfield, Samuel M. Young Jr. Ph.D., Samuel M. YoungJr., Samuel M. Young |
| Editors |
Steven D. Schwartzbach, Omar Skalli, Thomas Schikorski |
| Abstract |
Neuroscience research has been revolutionized by the use of recombinant viral vector technology from the basic, preclinical and clinical levels. Currently, multiple recombinant viral vector types are employed with each having its strengths and weaknesses depending on the proposed application. Helper-dependent adenoviral vectors (HdAd) are emerging as ideal viral vectors that solve a major need in the neuroscience field: (1) expression of transgenes that are too large to be packaged by other viral vectors and (2) rapid onset of transgene expression in the absence of cytotoxicity. Here, we describe the methods for large-scale production of HdAd viral vectors for in vivo use with neurospecific transgene expression. |
Mendeley readers
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 11 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 5 | 45% |
| Student > Ph. D. Student | 3 | 27% |
| Student > Bachelor | 2 | 18% |
| Professor | 1 | 9% |
| Readers by discipline | Count | As % |
|---|---|---|
| Neuroscience | 6 | 55% |
| Agricultural and Biological Sciences | 4 | 36% |
| Medicine and Dentistry | 1 | 9% |