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High-Resolution Imaging of Cellular Proteins

Overview of attention for book
Cover of 'High-Resolution Imaging of Cellular Proteins'

Table of Contents

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    Book Overview
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    Chapter 1 Expression of Epitope-Tagged Proteins in Mammalian Cells in Culture.
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    Chapter 2 Antibody Production with Synthetic Peptides.
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    Chapter 3 High-Resolution Imaging of Cellular Proteins
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    Chapter 4 Preparation of Colloidal Gold Particles and Conjugation to Protein A/G/L, IgG, F(ab')2, and Streptavidin.
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    Chapter 5 Helper-Dependent Adenoviral Vectors and Their Use for Neuroscience Applications.
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    Chapter 6 Localizing Proteins in Fixed Giardia lamblia and Live Cultured Mammalian Cells by Confocal Fluorescence Microscopy.
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    Chapter 7 Using Fluorescent Protein Fusions to Study Protein Subcellular Localization and Dynamics in Plant Cells.
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    Chapter 8 Using FRAP or FRAPA to Visualize the Movement of Fluorescently Labeled Proteins or Cellular Organelles in Live Cultured Neurons Transformed with Adeno-Associated Viruses.
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    Chapter 9 Bimolecular Fluorescence Complementation (BiFC) Analysis of Protein-Protein Interactions and Assessment of Subcellular Localization in Live Cells.
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    Chapter 10 Viral Injection and Cranial Window Implantation for In Vivo Two-Photon Imaging.
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    Chapter 11 Imaging Synaptic Vesicle Exocytosis-Endocytosis with pH-Sensitive Fluorescent Proteins.
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    Chapter 12 Immunogold Protein Localization on Grid-Glued Freeze-Fracture Replicas.
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    Chapter 13 Pre-embedding Double-Label Immunoelectron Microscopy of Chemically Fixed Tissue Culture Cells.
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    Chapter 14 Immunoelectron Microscopy of Cryofixed and Freeze-Substituted Plant Tissues.
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    Chapter 15 High-Resolution Imaging of Cellular Proteins
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    Chapter 16 Pre-embedding Method of Electron Microscopy for Glycan Localization in Mammalian Tissues and Cells Using Lectin Probes.
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    Chapter 17 Pre-embedding Nanogold Silver and Gold Intensification.
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    Chapter 18 Post-embedding Mammalian Tissue for Immunoelectron Microscopy: A Standardized Procedure Based on Heat-Induced Antigen Retrieval.
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    Chapter 19 Pre- and Post-embedding Immunogold Labeling of Tissue Sections.
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    Chapter 20 High-Resolution Imaging of Cellular Proteins
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    Chapter 21 Monitoring Synaptic Vesicle Protein Sorting with Enhanced Horseradish Peroxidase in the Electron Microscope.
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    Chapter 22 High-Resolution Imaging of Cellular Proteins
Attention for Chapter 15: High-Resolution Imaging of Cellular Proteins
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Chapter title
High-Resolution Imaging of Cellular Proteins
Chapter number 15
Book title
High-Resolution Imaging of Cellular Proteins
Published in
Methods in molecular biology, January 2016
DOI 10.1007/978-1-4939-6352-2_15
Pubmed ID
27515085
Book ISBNs
978-1-4939-6350-8, 978-1-4939-6352-2
Authors

Fišerová, Jindřiška, Richardson, Christine, Goldberg, Martin W, Jindřiška Fišerová, Christine Richardson, Martin W. Goldberg

Editors

Steven D. Schwartzbach, Omar Skalli, Thomas Schikorski

Abstract

Immunolabeling electron microscopy is a challenging technique with demands for perfect ultrastructural and antigen preservation. High-pressure freezing offers an excellent way to fix cellular structure. However, its use for immunolabeling has remained limited because of the low frequency of labeling due to loss of protein antigenicity or accessibility. Here we present a protocol for immunogold labeling of the yeast Saccharomyces cerevisiae that gives specific and multiple labeling while keeping the finest structural details. We use the protocol to reveal the organization of individual nuclear pore complex proteins and the position of transport factors in the yeast Saccharomyces cerevisiae in relation to actual transport events.

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X Demographics

X Demographics

The data shown below were collected from the profiles of 2 X users who shared this research output. Click here to find out more about how the information was compiled.
 Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 8 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 8 100%

Demographic breakdown

Readers by professional status Count As %
Researcher 2 25%
Student > Ph. D. Student 2 25%
Student > Doctoral Student 1 13%
Lecturer > Senior Lecturer 1 13%
Professor 1 13%
Other 0 0%
Unknown 1 13%
Readers by discipline Count As %
Agricultural and Biological Sciences 6 75%
Biochemistry, Genetics and Molecular Biology 1 13%
Unknown 1 13%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 2. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 17 August 2016.
All research outputs
#19,017,658
of 23,577,761 outputs
Outputs from Methods in molecular biology
#8,198
of 13,410 outputs
Outputs of similar age
#288,332
of 397,072 outputs
Outputs of similar age from Methods in molecular biology
#872
of 1,472 outputs
Altmetric has tracked 23,577,761 research outputs across all sources so far. This one is in the 11th percentile – i.e., 11% of other outputs scored the same or lower than it.
So far Altmetric has tracked 13,410 research outputs from this source. They receive a mean Attention Score of 3.4. This one is in the 24th percentile – i.e., 24% of its peers scored the same or lower than it.
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We're also able to compare this research output to 1,472 others from the same source and published within six weeks on either side of this one. This one is in the 26th percentile – i.e., 26% of its contemporaries scored the same or lower than it.

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