↓ Skip to main content
What is this page? Embed badge

High-Resolution Imaging of Cellular Proteins

Overview of attention for book
Cover of 'High-Resolution Imaging of Cellular Proteins'

Table of Contents

  1. Altmetric Badge
    Book Overview
  2. Altmetric Badge
    Chapter 1 Expression of Epitope-Tagged Proteins in Mammalian Cells in Culture.
  3. Altmetric Badge
    Chapter 2 Antibody Production with Synthetic Peptides.
  4. Altmetric Badge
    Chapter 3 High-Resolution Imaging of Cellular Proteins
  5. Altmetric Badge
    Chapter 4 Preparation of Colloidal Gold Particles and Conjugation to Protein A/G/L, IgG, F(ab')2, and Streptavidin.
  6. Altmetric Badge
    Chapter 5 Helper-Dependent Adenoviral Vectors and Their Use for Neuroscience Applications.
  7. Altmetric Badge
    Chapter 6 Localizing Proteins in Fixed Giardia lamblia and Live Cultured Mammalian Cells by Confocal Fluorescence Microscopy.
  8. Altmetric Badge
    Chapter 7 Using Fluorescent Protein Fusions to Study Protein Subcellular Localization and Dynamics in Plant Cells.
  9. Altmetric Badge
    Chapter 8 Using FRAP or FRAPA to Visualize the Movement of Fluorescently Labeled Proteins or Cellular Organelles in Live Cultured Neurons Transformed with Adeno-Associated Viruses.
  10. Altmetric Badge
    Chapter 9 Bimolecular Fluorescence Complementation (BiFC) Analysis of Protein-Protein Interactions and Assessment of Subcellular Localization in Live Cells.
  11. Altmetric Badge
    Chapter 10 Viral Injection and Cranial Window Implantation for In Vivo Two-Photon Imaging.
  12. Altmetric Badge
    Chapter 11 Imaging Synaptic Vesicle Exocytosis-Endocytosis with pH-Sensitive Fluorescent Proteins.
  13. Altmetric Badge
    Chapter 12 Immunogold Protein Localization on Grid-Glued Freeze-Fracture Replicas.
  14. Altmetric Badge
    Chapter 13 Pre-embedding Double-Label Immunoelectron Microscopy of Chemically Fixed Tissue Culture Cells.
  15. Altmetric Badge
    Chapter 14 Immunoelectron Microscopy of Cryofixed and Freeze-Substituted Plant Tissues.
  16. Altmetric Badge
    Chapter 15 High-Resolution Imaging of Cellular Proteins
  17. Altmetric Badge
    Chapter 16 Pre-embedding Method of Electron Microscopy for Glycan Localization in Mammalian Tissues and Cells Using Lectin Probes.
  18. Altmetric Badge
    Chapter 17 Pre-embedding Nanogold Silver and Gold Intensification.
  19. Altmetric Badge
    Chapter 18 Post-embedding Mammalian Tissue for Immunoelectron Microscopy: A Standardized Procedure Based on Heat-Induced Antigen Retrieval.
  20. Altmetric Badge
    Chapter 19 Pre- and Post-embedding Immunogold Labeling of Tissue Sections.
  21. Altmetric Badge
    Chapter 20 High-Resolution Imaging of Cellular Proteins
  22. Altmetric Badge
    Chapter 21 Monitoring Synaptic Vesicle Protein Sorting with Enhanced Horseradish Peroxidase in the Electron Microscope.
  23. Altmetric Badge
    Chapter 22 High-Resolution Imaging of Cellular Proteins
Attention for Chapter 13: Pre-embedding Double-Label Immunoelectron Microscopy of Chemically Fixed Tissue Culture Cells.
Altmetric Badge

Mentioned by

twitter
1 X user

Citations

dimensions_citation
4 Dimensions

Readers on

mendeley
13 Mendeley
Summary X Dimensions citations
You are seeing a free-to-access but limited selection of the activity Altmetric has collected about this research output. Click here to find out more.
Chapter title
Pre-embedding Double-Label Immunoelectron Microscopy of Chemically Fixed Tissue Culture Cells.
Chapter number 13
Book title
High-Resolution Imaging of Cellular Proteins
Published in
Methods in molecular biology, January 2016
DOI 10.1007/978-1-4939-6352-2_13
Pubmed ID
27515083
Book ISBNs
978-1-4939-6350-8, 978-1-4939-6352-2
Authors

Lou G. Boykins, Jonathan C. R. Jones, Carlos E. Estraño, Steven D. Schwartzbach, Omar Skalli

Editors

Steven D. Schwartzbach, Omar Skalli, Thomas Schikorski

Abstract

Localization of specific proteins within cells at the nanometer level of resolution is central to understanding how these proteins function in cell processes such as motility and intracellular trafficking. Such localization can be achieved by combining transmission electron microscopy (TEM) with immunogold labeling. Here we describe a pre-embedding, indirect gold immunolabeling approach to localize two different proteins of interest with secondary antibodies labeled with gold particles of different sizes in cells grown on cover slips. In this protocol, the cells are immunolabeled prior to being embedded in an epoxy resin for ultrathin sectioning. The protocol also includes strategies for optimizing the balance between ultrastructure and antigen preservation, steps to minimize nonspecific antibody binding, and steps to optimize antibody penetration.

View on publisher site
X Demographics

X Demographics

The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
 Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 13 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 13 100%

Demographic breakdown

Readers by professional status Count As %
Student > Bachelor 2 15%
Student > Ph. D. Student 2 15%
Researcher 2 15%
Unspecified 1 8%
Professor 1 8%
Other 3 23%
Unknown 2 15%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 4 31%
Neuroscience 2 15%
Medicine and Dentistry 2 15%
Environmental Science 1 8%
Unspecified 1 8%
Other 1 8%
Unknown 2 15%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 18 August 2016.
All research outputs
#18,467,727
of 22,883,326 outputs
Outputs from Methods in molecular biology
#7,923
of 13,131 outputs
Outputs of similar age
#284,560
of 393,707 outputs
Outputs of similar age from Methods in molecular biology
#845
of 1,471 outputs
Altmetric has tracked 22,883,326 research outputs across all sources so far. This one is in the 11th percentile – i.e., 11% of other outputs scored the same or lower than it.
So far Altmetric has tracked 13,131 research outputs from this source. They receive a mean Attention Score of 3.4. This one is in the 24th percentile – i.e., 24% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 393,707 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 15th percentile – i.e., 15% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 1,471 others from the same source and published within six weeks on either side of this one. This one is in the 28th percentile – i.e., 28% of its contemporaries scored the same or lower than it.

This page is provided by Altmetric.