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Mendeley readers
| Chapter title |
Preparation of Colloidal Gold Particles and Conjugation to Protein A/G/L, IgG, F(ab')2, and Streptavidin.
|
|---|---|
| Chapter number | 4 |
| Book title |
High-Resolution Imaging of Cellular Proteins
|
| Published in |
Methods in molecular biology, January 2016
|
| DOI | 10.1007/978-1-4939-6352-2_4 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-6350-8, 978-1-4939-6352-2
|
| Authors |
Sadaki Yokota |
| Editors |
Steven D. Schwartzbach, Omar Skalli, Thomas Schikorski |
| Abstract |
Colloidal gold probes, including protein A/G/L, IgG, F(ab')2, and streptavidin labeled with gold particles, are useful tools to localize antigens in cells and tissues by immunoelectron microscopy (IEM). This chapter describes different methods for the preparation of colloidal gold and conjugation of colloidal gold to protein A/G/L, IgG, and streptavidin. |
Mendeley readers
The data shown below were compiled from readership statistics for 11 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United States | 1 | 9% |
| Unknown | 10 | 91% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Master | 3 | 27% |
| Student > Ph. D. Student | 3 | 27% |
| Student > Doctoral Student | 1 | 9% |
| Student > Bachelor | 1 | 9% |
| Researcher | 1 | 9% |
| Other | 1 | 9% |
| Unknown | 1 | 9% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 4 | 36% |
| Chemistry | 3 | 27% |
| Veterinary Science and Veterinary Medicine | 1 | 9% |
| Medicine and Dentistry | 1 | 9% |
| Business, Management and Accounting | 1 | 9% |
| Other | 0 | 0% |
| Unknown | 1 | 9% |