Chapter title |
Lentiviral Vectors and Exosomes as Gene and Protein Delivery Tools
|
---|---|
Chapter number | 13 |
Book title |
Lentiviral Vectors and Exosomes as Gene and Protein Delivery Tools
|
Published in |
Methods in molecular biology, January 2016
|
DOI | 10.1007/978-1-4939-3753-0_13 |
Pubmed ID | |
Book ISBNs |
978-1-4939-3751-6, 978-1-4939-3753-0
|
Authors |
Lu-Nguyen, Ngoc B, Broadstock, Martin, Yáñez-Muñoz, Rafael J, Ngoc B. Lu-Nguyen, Martin Broadstock, Rafael J. Yáñez-Muñoz |
Editors |
Maurizio Federico |
Abstract |
Standard integration-proficient lentiviral vectors (IPLVs) are effective at much lower doses than other vector systems and have shown promise in several gene therapy approaches. Their main drawback is the potential risk of insertional mutagenesis. Novel biosafety-enhanced integration-deficient lentiviral vectors (IDLVs) offer a significant improvement and comparable transduction efficacy to their integrating counterparts in some central nervous system applications. We describe here methods for (1) production of IDLVs (and IPLVs), (2) IDLV/IPLV delivery into the striatum of a rat model of Parkinson's disease, and (3) postmortem brain processing. |
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