| Chapter title |
Development of Lentiviral Vectors for Targeted Integration and Protein Delivery.
|
|---|---|
| Chapter number | 14 |
| Book title |
Lentiviral Vectors and Exosomes as Gene and Protein Delivery Tools
|
| Published in |
Methods in molecular biology, January 2016
|
| DOI | 10.1007/978-1-4939-3753-0_14 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-3751-6, 978-1-4939-3753-0
|
| Authors |
Diana Schenkwein, Seppo Ylä-Herttuala |
| Editors |
Maurizio Federico |
| Abstract |
The method in this chapter describes the design of human immunodeficiency virus type 1 (HIV-1) integrase (IN)-fusion proteins which we have developed to transport different proteins into the nuclei of lentiviral vector (LV)-transduced cells. The IN-fusion protein cDNA is incorporated into the LV packaging plasmid, which leads to its incorporation into vector particles as part of a large Gag-Pol polyprotein. This specific feature of protein packaging enables also the incorporation of cytotoxic and proapoptotic proteins, such as frequently cutting endonucleases and P53. The vectors can hence be used for various protein transduction needs. An outline of the necessary methods is also given to study the functionality of a chosen IN-fusion protein in a cell culture assay. |
Mendeley readers
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 6 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Doctoral Student | 1 | 17% |
| Student > Bachelor | 1 | 17% |
| Professor | 1 | 17% |
| Student > Ph. D. Student | 1 | 17% |
| Researcher | 1 | 17% |
| Other | 0 | 0% |
| Unknown | 1 | 17% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 3 | 50% |
| Immunology and Microbiology | 1 | 17% |
| Neuroscience | 1 | 17% |
| Unknown | 1 | 17% |