Chapter title |
Production and Concentration of Lentivirus for Transduction of Primary Human T Cells.
|
---|---|
Chapter number | 7 |
Book title |
Lentiviral Vectors and Exosomes as Gene and Protein Delivery Tools
|
Published in |
Methods in molecular biology, January 2016
|
DOI | 10.1007/978-1-4939-3753-0_7 |
Pubmed ID | |
Book ISBNs |
978-1-4939-3751-6, 978-1-4939-3753-0
|
Authors |
Alan Kennedy, Adam P. Cribbs |
Editors |
Maurizio Federico |
Abstract |
Lentiviral vectors have emerged as efficient tools for investigating T cell biology through their ability to efficiently deliver transgene expression into both dividing and nondividing cells. Such lentiviral vectors have the potential to infect a wide variety of cell types. However, despite this advantage, the ability to transduce primary human T cells remains challenging and methods to achieve efficient gene transfer are often time consuming and expensive. We describe a method for generating lentivirus that is simple to perform and does not require the purchase of non-standard equipment to transduce primary human T cells. Therefore, we provide an optimized protocol that is easy to implement and allow transduction with high efficiency and reproducibility. |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 29 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Ph. D. Student | 6 | 21% |
Researcher | 5 | 17% |
Student > Doctoral Student | 3 | 10% |
Student > Master | 3 | 10% |
Student > Bachelor | 2 | 7% |
Other | 3 | 10% |
Unknown | 7 | 24% |
Readers by discipline | Count | As % |
---|---|---|
Biochemistry, Genetics and Molecular Biology | 10 | 34% |
Immunology and Microbiology | 4 | 14% |
Agricultural and Biological Sciences | 4 | 14% |
Chemical Engineering | 2 | 7% |
Medicine and Dentistry | 2 | 7% |
Other | 1 | 3% |
Unknown | 6 | 21% |