| Chapter title |
Transduction of Murine Hematopoietic Stem Cells with Tetracycline-regulated Lentiviral Vectors.
|
|---|---|
| Chapter number | 5 |
| Book title |
Lentiviral Vectors and Exosomes as Gene and Protein Delivery Tools
|
| Published in |
Methods in molecular biology, January 2016
|
| DOI | 10.1007/978-1-4939-3753-0_5 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-3751-6, 978-1-4939-3753-0
|
| Authors |
Maike Stahlhut, Axel Schambach, Olga S. Kustikova Ph.D., Olga S. Kustikova |
| Editors |
Maurizio Federico |
| Abstract |
Tetracycline-regulated integrating vectors allow pharmacologically controlled genetic modification of murine and human hematopoietic stem cells (HSCs). This approach combines the stable transgene insertion into a host genome with the opportunity for time- and dose-controlled reversible transgene expression in HSCs. Here, we describe the step-by-step protocol for transduction of murine stem-cell enriched populations of bone marrow cells, such as lineage negative cells (Lin(-)), with a lentiviral vector expressing the enhanced green fluorescent protein (EGFP) under the control of the tetracycline-regulated promoter. This chapter explains how to establish in vitro and in vivo systems to study transgene dose-dependent mechanisms affecting cell fate decisions of genetically modified hematopoietic cells. |
Mendeley readers
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 9 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Other | 2 | 22% |
| Student > Bachelor | 2 | 22% |
| Student > Doctoral Student | 1 | 11% |
| Professor | 1 | 11% |
| Student > Ph. D. Student | 1 | 11% |
| Other | 1 | 11% |
| Unknown | 1 | 11% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 2 | 22% |
| Medicine and Dentistry | 2 | 22% |
| Neuroscience | 1 | 11% |
| Nursing and Health Professions | 1 | 11% |
| Unknown | 3 | 33% |