| Chapter title |
TALENs
|
|---|---|
| Chapter number | 11 |
| Book title |
TALENs
|
| Published in |
Methods in molecular biology, January 2016
|
| DOI | 10.1007/978-1-4939-2932-0_11 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-2931-3, 978-1-4939-2932-0
|
| Authors |
Takasu, Yoko, Tamura, Toshiki, Goldsmith, Marian, Zurovec, Michal, Yoko Takasu, Toshiki Tamura, Marian Goldsmith, Michal Zurovec |
| Abstract |
Bombyx mori is a valuable model organism of high economic importance. Its genome sequence is available, as well as basic genetic and molecular genetic tools and markers. The introduction of genome editing methods based on engineered nucleases enables precise manipulations with genomic DNA, including targeted DNA deletions, insertions, or replacements in the genome allowing gene analysis and various applications. We describe here the use of TALENs which have a simple modular design of their DNA-binding domains, are easy to prepare and proved to be efficient in targeting of a wide range of cleavage sites. Our procedure often allows the production of individuals carrying homozygous mutations as early as in the G1 generation. |
X Demographics
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| Unknown | 1 | 100% |
Demographic breakdown
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|---|---|---|
| Members of the public | 1 | 100% |
Mendeley readers
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 5 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Other | 1 | 20% |
| Student > Doctoral Student | 1 | 20% |
| Professor | 1 | 20% |
| Student > Master | 1 | 20% |
| Researcher | 1 | 20% |
| Other | 0 | 0% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 5 | 100% |