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Mendeley readers
| Chapter title |
Assembly of Customized TAL Effectors Through Advanced ULtiMATE System
|
|---|---|
| Chapter number | 5 |
| Book title |
TALENs
|
| Published in |
Methods in molecular biology, January 2016
|
| DOI | 10.1007/978-1-4939-2932-0_5 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-2931-3, 978-1-4939-2932-0
|
| Authors |
Junjiao Yang, Shengjie Guo, Pengfei Yuan, Wensheng Wei, Yang, Junjiao, Guo, Shengjie, Yuan, Pengfei, Wei, Wensheng |
| Abstract |
Transcription activator-like effectors (TALEs) have been widely applied in gene targeting. Here we describe an advanced ULtiMATE (USER-based Ligation-Mediated Assembly of TAL Effector) system that utilizes USER fusion technique and archive of 512 tetramer templates to achieve highly efficient construction of TALEs, which takes only half a day to accomplish the assembly of any given TALE construct. This system is also suitable for large-scale assembly of TALENs and any other TALE-based constructions. |
Mendeley readers
The data shown below were compiled from readership statistics for 4 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Slovenia | 1 | 25% |
| Unknown | 3 | 75% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 2 | 50% |
| Student > Bachelor | 1 | 25% |
| Unknown | 1 | 25% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 2 | 50% |
| Biochemistry, Genetics and Molecular Biology | 1 | 25% |
| Unknown | 1 | 25% |